Detailed information of TA system TA242201

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 75024..75559
• Replicon: plasmid p2350-1
• Accession: NZ_CP095519
• Organism: Escherichia coli strain GN02350

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A0M1UJC2
• Locus tag: MW405_RS25315
• Protein ID: WP_001270422.1
• Coordinates: 75024..75311 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: L4J0V6
• Locus tag: MW405_RS25320
• Protein ID: WP_001132895.1
• Coordinates: 75308..75559 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MW405_RS25290 (70162)	70162..71331	-	1170	WP_001072358.1	hypothetical protein	-
MW405_RS25295 (71698)	71698..71886	+	189	WP_001332784.1	hypothetical protein	-
MW405_RS25300 (72007)	72007..72747	+	741	WP_001066947.1	tyrosine-type recombinase/integrase	-
MW405_RS25305 (72990)	72990..73967	-	978	WP_001309253.1	RepB family plasmid replication initiator protein	-
MW405_RS25310 (74611)	74611..75022	-	412	Protein_78	DDE-type integrase/transposase/recombinase	-
MW405_RS25315 (75024)	75024..75311	-	288	WP_001270422.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MW405_RS25320 (75308)	75308..75559	-	252	WP_001132895.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
MW405_RS25325 (75732)	75732..75863	-	132	WP_001309255.1	hypothetical protein	-
MW405_RS25330 (76317)	76317..77522	+	1206	WP_000817635.1	AAA family ATPase	-
MW405_RS25335 (77519)	77519..78481	+	963	WP_000756328.1	ParB family protein	-
MW405_RS25340 (78715)	78715..79491	+	777	Protein_84	transposase DNA-binding-containing protein	-
MW405_RS25345 (79621)	79621..79704	-	84	Protein_85	DUF4113 domain-containing protein	-
MW405_RS25350 (79706)	79706..80234	+	529	Protein_86	DUF1281 domain-containing protein	-
MW405_RS25355 (80231)	80231..80542	+	312	WP_001309256.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	ant(3'')-Ia / sul1 / blaSHV-48	senB	1..117950	117950
	-	flank	IS/Tn	-	-	79138..79278	140

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11262.30 Da
• Isoelectric Point: 10.4703

>T242201 WP_001270422.1 NZ_CP095519:c75311-75024 [Escherichia coli]
MRYQVKFREDALKEWQKMDKTIQQQFAKKLKKCCDNPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDEQLIIAVVAVGK
RERSDVYNLASERMR

Nucleotide

• Length: 288 bp

>T242201 NZ_OW052189:c495485-495090 [Mycobacterium tuberculosis]
GTGATCGTCGATACGTCGGCCATCGTCGCCATCGTGAGCGGGGAATCGGGCGCGCAGGTGCTCAAGGAGGCGCTGGAGCG
GTCACCGAACTCCCGAATGTCCGCGCCCAACTACGTCGAACTGTGCGCGATCATGCAGCGGCGGGACCGGCCGGAGATCT
CTCGATTGGTGGACCGTTTGCTGGACGACTACGGAATCCAGGTCGAAGCCGTCGACGCCGACCAAGCCCGCGTGGCCGCG
CAGGCGTATCGCGACTACGGCCGCGGCAGCGGCCATCCGGCCCGTCTCAACCTCGGCGACACCTACAGCTACGCCCTGGC
CCAGGTCACCGGGGAGCCGCTGCTGTTTCGTGGCGATGACTTCACGCATACCGATATCCGGCCCGCGTGCACCTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9178.47 Da
• Isoelectric Point: 4.2108

>AT242201 WP_001132895.1 NZ_CP095519:c75559-75308 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGFPVAILNRNQPAFYCVPAELYEKMLDALDDQELVKLVAERSNQPLHDVDLD
KYL

Nucleotide

• Length: 252 bp

>AT242201 NZ_OW052189:c495064-494798 [Mycobacterium tuberculosis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Structures

Toxin

• AlphaFold DB: A0A0M1UJC2

Antitoxin

• AlphaFold DB: L4J0V6