Detailed information of TA system TA024220

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 947401..947960
• Replicon: chromosome
• Accession: NC_013119
• Organism: Brucella microti CCM 4915

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: D6LP74
• Locus tag: BMI_RS04550
• Protein ID: WP_004688321.1
• Coordinates: 947715..947960 (-)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q57DF1
• Locus tag: BMI_RS04545
• Protein ID: WP_002964097.1
• Coordinates: 947401..947715 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BMI_RS04500	943091..943294	-	204	WP_002964087.1	hypothetical protein	-
BMI_RS04505	943297..943512	-	216	WP_002971459.1	hypothetical protein	-
BMI_RS04510	943509..943712	-	204	WP_002964088.1	hypothetical protein	-
BMI_RS04515	943762..944481	+	720	WP_041594692.1	hypothetical protein	-
BMI_RS04520	944478..944708	+	231	WP_002964091.1	hypothetical protein	-
BMI_RS04525	944705..944980	+	276	WP_002964092.1	hypothetical protein	-
BMI_RS04530	945003..945590	+	588	WP_004683738.1	hypothetical protein	-
BMI_RS04535	945825..946535	+	711	WP_004683739.1	porin family protein	-
BMI_RS04540	946883..947053	-	171	WP_002964095.1	DUF1508 domain-containing protein	-
BMI_RS04545	947401..947715	-	315	WP_002964097.1	BrnA antitoxin family protein	Antitoxin
BMI_RS04550	947715..947960	-	246	WP_004688321.1	BrnT family toxin	Toxin
BMI_RS04555	948093..948731	+	639	Protein_889	tRNA-guanine transglycosylase	-
BMI_RS17295	948801..949559	+	759	Protein_890	IS5 family transposase	-
BMI_RS04570	949898..950332	+	435	WP_006137279.1	hypothetical protein	-
BMI_RS04575	950467..952113	+	1647	WP_004689673.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	wboA / wbdA	941319..955429	14110
	-	flank	IS/Tn	-	-	948801..949169	368

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-78)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-102)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9172.86 Da
• Isoelectric Point: 10.3158

>T24220 WP_004688321.1 NC_013119:c947960-947715 [Brucella microti CCM 4915]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRPASQKERK
L

Nucleotide

• Length: 246 bp

>T24220 NC_013119:c947960-947715 [Brucella microti CCM 4915]
ATGAAGATCATCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCCTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCATTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTCCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11638.42 Da
• Isoelectric Point: 10.3131

>AT24220 WP_002964097.1 NC_013119:c947715-947401 [Brucella microti CCM 4915]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDEQLAKAKPFKEAFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Nucleotide

• Length: 315 bp

>AT24220 NC_013119:c947715-947401 [Brucella microti CCM 4915]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAAGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATGAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAAGCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCCGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA