Detailed information of TA system TA242196

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 4845374..4846962
• Replicon: chromosome
• Accession: NZ_CP095518
• Organism: Escherichia coli strain GN02350

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: B7MMY1
• Locus tag: MW405_RS23025
• Protein ID: WP_001125475.1
• Coordinates: 4845640..4846962 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: MW405_RS23020
• Protein ID: WP_001332145.1
• Coordinates: 4845374..4845640 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MW405_RS23005 (4841742)	4841742..4843193	+	1452	WP_000854636.1	tagaturonate reductase	-
MW405_RS23010 (4843390)	4843390..4844304	+	915	WP_020231438.1	bestrophin family protein	-
MW405_RS23015 (4844308)	4844308..4845066	-	759	WP_001286588.1	trans-aconitate 2-methyltransferase	-
MW405_RS23020 (4845374)	4845374..4845640	+	267	WP_001332145.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
MW405_RS23025 (4845640)	4845640..4846962	+	1323	WP_001125475.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
MW405_RS23030 (4847221)	4847221..4847882	-	662	Protein_4493	alpha/beta hydrolase	-
MW405_RS23035 (4847871)	4847871..4848350	+	480	WP_015912499.1	cupin domain-containing protein	-
MW405_RS23040 (4848328)	4848328..4849089	+	762	WP_001066622.1	carboxymuconolactone decarboxylase family protein	-
MW405_RS23045 (4849247)	4849247..4850311	+	1065	WP_000859696.1	alpha/beta fold hydrolase	-
MW405_RS23050 (4850560)	4850560..4851032	-	473	Protein_4497	LysR substrate-binding domain-containing protein	-
MW405_RS23055 (4851169)	4851169..4851951	+	783	WP_000699097.1	SDR family oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49426.24 Da
• Isoelectric Point: 8.9380

>T242196 WP_001125475.1 NZ_CP095518:4845640-4846962 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRRNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVMCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVKRFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIAQIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Nucleotide

• Length: 1323 bp

>T242196 NZ_OW052189:294187-294564 [Mycobacterium tuberculosis]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10016.44 Da
• Isoelectric Point: 6.2267

>AT242196 WP_001332145.1 NZ_CP095518:4845374-4845640 [Escherichia coli]
MMSFQKIYSPMQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPNNTSLTTFFKILQSLELSMTLCDAKNASPEA
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT242196 NZ_OW052189:293933-294190 [Mycobacterium tuberculosis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Structures

Toxin

• AlphaFold DB: A0A0E2L5M8