Detailed information of TA system TA242178

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1643008..1643840
• Replicon: chromosome
• Accession: NZ_CP095518
• Organism: Escherichia coli strain GN02350

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A2R9V8K2
• Locus tag: MW405_RS07805
• Protein ID: WP_001468432.1
• Coordinates: 1643466..1643840 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: Q1R2L5
• Locus tag: MW405_RS07800
• Protein ID: WP_001285575.1
• Coordinates: 1643008..1643376 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MW405_RS07765 (1638901)	1638901..1639581	+	681	WP_001282919.1	WYL domain-containing protein	-
MW405_RS07770 (1639729)	1639729..1640406	+	678	WP_001097301.1	hypothetical protein	-
MW405_RS07775 (1640412)	1640412..1640645	+	234	WP_001278283.1	DUF905 family protein	-
MW405_RS07780 (1640735)	1640735..1641553	+	819	WP_001175160.1	DUF932 domain-containing protein	-
MW405_RS07785 (1641644)	1641644..1642129	+	486	WP_000213697.1	antirestriction protein	-
MW405_RS07790 (1642144)	1642144..1642620	+	477	WP_001298074.1	RadC family protein	-
MW405_RS07795 (1642707)	1642707..1642928	+	222	WP_000692350.1	DUF987 domain-containing protein	-
MW405_RS07800 (1643008)	1643008..1643376	+	369	WP_001285575.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
MW405_RS07805 (1643466)	1643466..1643840	+	375	WP_001468432.1	TA system toxin CbtA family protein	Toxin
MW405_RS07810 (1643837)	1643837..1644325	+	489	WP_000777545.1	DUF5983 family protein	-
MW405_RS07815 (1644337)	1644337..1644534	+	198	WP_000839281.1	DUF957 domain-containing protein	-
MW405_RS07820 (1644631)	1644631..1645200	+	570	WP_001290252.1	DUF4942 domain-containing protein	-
MW405_RS07825 (1645949)	1645949..1647487	+	1539	WP_001187191.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1624581..1655300	30719

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14102.04 Da
• Isoelectric Point: 7.3526

>T242178 WP_001468432.1 NZ_CP095518:1643466-1643840 [Escherichia coli]
MNTLPDTHIREASHCQSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPEKR

Nucleotide

• Length: 375 bp

>T242178 NZ_OW052188:3516938-3517372 [Mycobacterium tuberculosis]
ATGAAGATCGTCGACGCGAACGTCTTGCTCTACGCCGTGAACACCACAAGTGAGCACCACAAGCCGTCGCTGCGCTGGCT
TGACGGTGCGCTGTCGGGCGCCGACCGCGTCGGGTTCGCCTGGGTGCCGTTGTTGGCGTTCGTGCGATTGGCGACCAAGG
TGGGGTTGTTCCCCCGTCCGCTTCCGCGGGAGGCGGCCATCACCCAGGTCGCGGATTGGCTAGCCGCACCCAGCGCCGTC
TTGGTGAATCCGACCGTCCGGCACGCCGATATCCTGGCGAGAATGCTGACGTACGTGGGAACCGGTGCCAACCTGGTCAA
CGACGCGCATCTGGCCGCGCTTGCCGTCGAGCATCGCGCCAGCATCGTGTCCTACGACAGTGACTTCGGCCGATTCGAAG
GGGTGCGCTGGGATCAGCCGCCCGCGCTGTTGTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13709.52 Da
• Isoelectric Point: 6.6258

>AT242178 WP_001285575.1 NZ_CP095518:1643008-1643376 [Escherichia coli]
VSDTLPGTTHPDDNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKRLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT242178 NZ_OW052188:3516677-3516931 [Mycobacterium tuberculosis]
ATGCGGACGACTGTCACATTGGATGACGACGTCGAGCAGTTGGTAAGGCGCCGGATGGCCGAGCGGCAAGTGTCCTTCAA
GAAGGCCCTCAACGACGCGATTCGCGACGGCGCCTCCGGGCGACCTGCGCCGAGTCACTTCAGCACCCGCACGGCAGACT
TGGGCGTCCCTGCGGTCAACCTCGACCGGGCCTTGCAGCTTGCGGCCGACCTCGAGGACGAAGAGCTTGTGCGCCGTCAA
CGCCGCGGGTCATAG

Structures

Toxin

• AlphaFold DB: A0A2R9V8K2

Antitoxin

• AlphaFold DB: A0A454ABF5