Detailed information of TA system TA242170

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 1176982..1177532
• Replicon: chromosome
• Accession: NZ_CP095518
• Organism: Escherichia coli strain GN02350

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: S1PDG4
• Locus tag: MW405_RS05570
• Protein ID: WP_001160966.1
• Coordinates: 1176982..1177296 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V0VEX8
• Locus tag: MW405_RS05575
• Protein ID: WP_000125566.1
• Coordinates: 1177299..1177532 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MW405_RS05545 (1172657)	1172657..1173943	+	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-
MW405_RS05550 (1173943)	1173943..1174932	+	990	WP_000241229.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
MW405_RS05555 (1174929)	1174929..1175750	+	822	WP_001065363.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
MW405_RS05560 (1175753)	1175753..1176130	+	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
MW405_RS05565 (1176137)	1176137..1176985	+	849	WP_000257182.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
MW405_RS05570 (1176982)	1176982..1177296	-	315	WP_001160966.1	CcdB family protein	Toxin
MW405_RS05575 (1177299)	1177299..1177532	-	234	WP_000125566.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
MW405_RS05580 (1177618)	1177618..1178097	-	480	WP_000624375.1	type 3 dihydrofolate reductase	-
MW405_RS05585 (1178289)	1178289..1180151	-	1863	WP_020231452.1	glutathione-regulated potassium-efflux system protein KefC	-
MW405_RS05590 (1180144)	1180144..1180674	-	531	WP_000600725.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
MW405_RS05595 (1180782)	1180782..1182113	-	1332	WP_001183207.1	MFS transporter	-
MW405_RS05600 (1182172)	1182172..1182459	-	288	WP_000203741.1	ferredoxin-like protein FixX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11711.72 Da
• Isoelectric Point: 9.0851

>T242170 WP_001160966.1 NZ_CP095518:c1177296-1176982 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Nucleotide

• Length: 315 bp

>T242170 NZ_OW052188:2823693-2824109 [Mycobacterium tuberculosis]
ATGACCACGCGCTATTTGCTCGACAAATCAGCGGCTTACCGCGCGCACTTGCCCGCGGTCCGACATCGCTTGGAACCGTT
GATGGAACGCGGTCTACTGGCCCGGTGCGGCATTACCGATCTCGAGTTCGGAGTCTCGGCGCGTTCCCGCGAGGACCATC
GAACACTGGGCACCTACCGGCGTGACGCGCTCGAATACGTCAACACCCCCGACACCGTGTGGGTTCGTGCATGGGAGATC
CAAGAAGCATTGACCGACAAGGGATTTCACCGCTCTGTCAAGATCCCGGACTTGATCATTGCGGCGGTCGCCGAGCATCA
CGGCATACCCGTCATGCACTACGACCAAGACTTCGAACGCATCGCCGCCATCACACGGCAACCCGTGGAATGGGTGGTGG
CCCCAGGGACAGCGTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8530.53 Da
• Isoelectric Point: 5.8062

>AT242170 WP_000125566.1 NZ_CP095518:c1177532-1177299 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT242170 NZ_OW052188:2823430-2823696 [Mycobacterium tuberculosis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Structures

Toxin

• AlphaFold DB: A0A829G815

Antitoxin

• AlphaFold DB: A0A7U9LTM9