Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 753292..753910 Replicon chromosome
Accession NZ_CP095518
Organism Escherichia coli strain GN02350

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag MW405_RS03610 Protein ID WP_001291435.1
Coordinates 753692..753910 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag MW405_RS03605 Protein ID WP_000344800.1
Coordinates 753292..753666 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MW405_RS03595 (748382) 748382..749575 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
MW405_RS03600 (749598) 749598..752747 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
MW405_RS03605 (753292) 753292..753666 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
MW405_RS03610 (753692) 753692..753910 + 219 WP_001291435.1 HHA domain-containing protein Toxin
MW405_RS03615 (754083) 754083..754634 + 552 WP_000102539.1 maltose O-acetyltransferase -
MW405_RS03620 (754750) 754750..755220 + 471 WP_000136192.1 YlaC family protein -
MW405_RS03625 (755384) 755384..756934 + 1551 WP_001468081.1 cyclic-guanylate-specific phosphodiesterase PdeB -
MW405_RS03630 (756976) 756976..757329 - 354 WP_000878142.1 DUF1428 family protein -
MW405_RS03640 (757708) 757708..758019 + 312 WP_000409908.1 MGMT family protein -
MW405_RS03645 (758050) 758050..758622 - 573 WP_000779844.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T242169 WP_001291435.1 NZ_CP095518:753692-753910 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T242169 NZ_OW052188:2823009-2823404 [Mycobacterium tuberculosis]
GTGATCGTCGATACGTCGGCCATCGTCGCCATCGTGAGCGGGGAATCGGGCGCGCAGGTGCTCAAGGAGGCGCTGGAGCG
GTCACCGAACTCCCGAATGTCCGCGCCCAACTACGTCGAACTGTGCGCGATCATGCAGCGGCGGGACCGGCCGGAGATCT
CTCGATTGGTGGACCGTTTGCTGGACGACTACGGAATCCAGGTCGAAGCCGTCGACGCCGACCAAGCCCGCGTGGCCGCG
CAGGCGTATCGCGACTACGGCCGCGGCAGCGGCCATCCGGCCCGTCTCAACCTCGGCGACACCTACAGCTACGCCCTGGC
CCAGGTCACCGGGGAGCCGCTGCTGTTTCGTGGCGATGACTTCACGCATACCGATATCCGGCCCGCGTGCACCTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT242169 WP_000344800.1 NZ_CP095518:753292-753666 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT242169 NZ_OW052188:2823430-2823696 [Mycobacterium tuberculosis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References