Detailed information of TA system TA242168

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 98133..98934
• Replicon: chromosome
• Accession: NZ_CP095518
• Organism: Escherichia coli strain GN02350

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0H2V687
• Locus tag: MW405_RS00525
• Protein ID: WP_000854739.1
• Coordinates: 98133..98513 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A0P7R0L9
• Locus tag: MW405_RS00530
• Protein ID: WP_001285482.1
• Coordinates: 98560..98934 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MW405_RS00490 (93725)	93725..94279	-	555	WP_001001921.1	molecular chaperone YcdY	-
MW405_RS00495 (94303)	94303..95040	-	738	WP_000283664.1	zinc-binding phosphatase	-
MW405_RS00500 (95095)	95095..96033	-	939	WP_000351277.1	glyoxylate/hydroxypyruvate reductase GhrA	-
MW405_RS00510 (96503)	96503..97346	-	844	Protein_100	DUF4942 domain-containing protein	-
MW405_RS00515 (97431)	97431..97628	-	198	WP_000772027.1	DUF957 domain-containing protein	-
MW405_RS00520 (97648)	97648..98136	-	489	WP_001054232.1	DUF5983 family protein	-
MW405_RS00525 (98133)	98133..98513	-	381	WP_000854739.1	TA system toxin CbtA family protein	Toxin
MW405_RS00530 (98560)	98560..98934	-	375	WP_001285482.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
MW405_RS00535 (98984)	98984..99628	-	645	WP_000086763.1	hypothetical protein	-
MW405_RS00540 (99647)	99647..99868	-	222	WP_000692315.1	DUF987 domain-containing protein	-
MW405_RS00545 (99931)	99931..100407	-	477	WP_001186170.1	RadC family protein	-
MW405_RS00550 (100423)	100423..100896	-	474	WP_000855076.1	antirestriction protein	-
MW405_RS00555 (101159)	101159..101980	-	822	WP_001234710.1	DUF932 domain-containing protein	-
MW405_RS00560 (102159)	102159..102248	-	90	WP_112031555.1	DUF905 family protein	-
MW405_RS00565 (102391)	102391..102846	-	456	WP_000581502.1	IrmA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 14214.14 Da
• Isoelectric Point: 6.4773

>T242168 WP_000854739.1 NZ_CP095518:c98513-98133 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGVPSQLINSIDILRARRATGLMTRDNYRTVNDITLGRHPEEAKQ

Nucleotide

• Length: 381 bp

>T242168 NZ_OW052188:2783754-2784110 [Mycobacterium tuberculosis]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13791.44 Da
• Isoelectric Point: 4.7511

>AT242168 WP_001285482.1 NZ_CP095518:c98934-98560 [Escherichia coli]
VSDTLHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTLAPATTS

Nucleotide

• Length: 375 bp

>AT242168 NZ_OW052188:2783540-2783770 [Mycobacterium tuberculosis]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Structures

Toxin

• AlphaFold DB: A0A0H2V687

Antitoxin

• AlphaFold DB: A0A0P7R0L9