Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 87679..88204 Replicon plasmid p2323-1
Accession NZ_CP095515
Organism Escherichia coli strain GN02323

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag MW403_RS24720 Protein ID WP_001159871.1
Coordinates 87679..87984 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag MW403_RS24725 Protein ID WP_000813634.1
Coordinates 87986..88204 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MW403_RS24690 (82946) 82946..84216 + 1271 Protein_102 translesion error-prone DNA polymerase V subunit UmuC -
MW403_RS24695 (84221) 84221..84613 - 393 WP_000340835.1 plasmid partitioning/stability family protein -
MW403_RS24700 (84618) 84618..85589 - 972 WP_001103694.1 plasmid segregation protein ParM -
MW403_RS24705 (85818) 85818..86462 + 645 WP_000633911.1 AAA family ATPase -
MW403_RS24710 (86456) 86456..86731 + 276 WP_000239529.1 hypothetical protein -
MW403_RS24715 (86869) 86869..87678 - 810 WP_000016979.1 site-specific integrase -
MW403_RS24720 (87679) 87679..87984 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
MW403_RS24725 (87986) 87986..88204 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
MW403_RS24730 (90526) 90526..91167 + 642 WP_000990667.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - senB 1..114242 114242


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T242161 WP_001159871.1 NZ_CP095515:c87984-87679 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T242161 NZ_OW052188:c2117983-2117726 [Mycobacterium tuberculosis]
GTGAGAAGCGTCAACTTCGATCCCGATGCCTGGGAGGACTTCTTGTTCTGGCTGGCCGCTGATCGCAAAACGGCCCGTCG
GATCACCCGGTTGATCGGAGAAATTCAGCGTGATCCGTTCAGCGGGATCGGCAAACCCGAGCCGCTCCAAGGTGAGTTGT
CGGGATACTGGTCGCGCCGGATCGACGACGAACACCGGCTGGTGTATCGAGCGGGCGACGACGAAGTCACGATGCTGAAG
GCCCGATACCACTACTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT242161 WP_000813634.1 NZ_CP095515:c88204-87986 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT242161 NZ_OW052188:c2118255-2117980 [Mycobacterium tuberculosis]
ATGAGCATCAGTGCGAGCGAGGCGAGGCAGCGCCTGTTTCCACTCATCGAACAGGTCAATACCGATCACCAGCCGGTGCG
GATCACCTCCCGGGCCGGCGATGCGGTGCTGATGTCCGCCGACGACTACGACGCGTGGCAGGAAACGGTCTATCTGCTGC
GCTCACCGGAGAACGCCAGGCGGTTGATGGAAGCGGTTGCCCGGGATAAGGCTGGGCACTCGGCTTTCACCAAGTCTGTA
GATGAGCTGCGGGAGATGGCCGGCGGCGAGGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References