Detailed information of TA system TA242160

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yeeU/CbtA-CbeA
• Location: 5027396..5028187
• Replicon: chromosome
• Accession: NZ_CP095514
• Organism: Escherichia coli strain GN02323

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: MW403_RS24045
• Protein ID: WP_000691791.1
• Coordinates: 5027762..5028187 (+)
• Length: 142 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A3L1KVZ6
• Locus tag: MW403_RS24040
• Protein ID: WP_000939437.1
• Coordinates: 5027396..5027731 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MW403_RS24015 (5023347)	5023347..5023565	-	219	WP_000070772.1	AlpA family phage regulatory protein	-
MW403_RS24020 (5023683)	5023683..5024297	-	615	WP_000772910.1	inovirus Gp2 family protein	-
MW403_RS24025 (5024890)	5024890..5025843	+	954	WP_000290406.1	hypothetical protein	-
MW403_RS24030 (5026079)	5026079..5026893	+	815	Protein_4693	DUF932 domain-containing protein	-
MW403_RS24035 (5026923)	5026923..5027396	+	474	WP_001292742.1	DNA repair protein RadC	-
MW403_RS24040 (5027396)	5027396..5027731	+	336	WP_000939437.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
MW403_RS24045 (5027762)	5027762..5028187	+	426	WP_000691791.1	TA system toxin CbtA family protein	Toxin
MW403_RS24050 (5028208)	5028208..5029399	+	1192	Protein_4697	AAA family ATPase	-
MW403_RS24055 (5029384)	5029384..5029605	+	222	WP_125112077.1	hypothetical protein	-
MW403_RS24060 (5029602)	5029602..5030333	+	732	WP_001216639.1	retron system putative HNH endonuclease	-
MW403_RS24065 (5030956)	5030956..5031342	+	387	WP_001270938.1	DUF2441 domain-containing protein	-
MW403_RS24070 (5032024)	5032024..5032521	+	498	WP_000509808.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	5006734..5032521	25787

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-108)

Antitoxin

• Antitoxin CbeA (10-110)

Sequences

Toxin

Protein

• Length: 142 a.a.
• Molecular weight: 16490.11 Da
• Isoelectric Point: 10.2376

>T242160 WP_000691791.1 NZ_CP095514:5027762-5028187 [Escherichia coli]
MKIIPATTLRATTSYLSPVVVWQTLLARLLEQHYGLNLNDTPFNNEKVIQEHIDAGITLVNAVNFLVEKYELVRIDRKGF
SWQEQTPYLRAVDILRARQATGLRIQCKNTNIISRMADSRHDKRLYQRTFKACKRCYYTKT

Nucleotide

• Length: 426 bp

>T242160 NZ_OW052188:2090740-2091132 [Mycobacterium tuberculosis]
ATGGCGGCCATCTATCTCGACTCGTCCGCGATCGTCAAGCTCGCGGTTCGCGAGCCAGAGTCGGACGCGCTGCGCCGCTA
CCTTCGCACCCGCCACCCGCGAGTGTCGAGTGCCCTTGCGCGCGCGGAGGTCATGCGGGCCCTGCTTGACAAGGGTGAAT
CAGCGCGGAAAGCCGGCCGCCGGGCGCTGGCTCATCTCGATCTGCTGCGCGTCGACAAGCGCGTCCTCGATCTCGCTGGT
GGGCTGCTGCCATTCGAGCTGCGCACGCTCGATGCCATCCACTTGGCGACGGCGCAGCGGCTCGGCGTCGATCTGGGCCG
GTTGTGCACCTACGACGACCGGATGCGCGACGCCGCCAAGACGCTCGGCATGGCCGTGATCGCCCCGTCGTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12556.56 Da
• Isoelectric Point: 7.6977

>AT242160 WP_000939437.1 NZ_CP095514:5027396-5027731 [Escherichia coli]
MLQKDKVMQQEWGLPCTVTPRFTARLVQERHNLHYLADRAAITGKFSSPDLLHIDQAFPVLMKQLELKLADGELSPCQQR
TVTLYAKGLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 336 bp

>AT242160 NZ_OW052188:2090465-2090740 [Mycobacterium tuberculosis]
ATGACCTCGGTGGGGGTGCGTGCGCTGCGGCAGCGGGCCAGCGAGCTGCTGCGCCGAGTCGAGGCCGGCGAGACGATCGA
GATCACCGACCGCGGCCGGCCCGTCGCCCTGCTCTCGCCGCTGCCGCAGGGCGGTCCATACGAGCAGCTGCTGGCAAGCG
GCGAGATCGAGCGTGCCACGCTCGACGTCGTTGATCTGCCCGAACCGCTCGACCTCGACGCGGGCGTCGAGCTGCCGTCG
GTGACGCTTGCGCGCCTGCGTGAGCACGAGCGTTGA

Structures

Antitoxin

• AlphaFold DB: A0A3L1KVZ6