Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3981723..3982377 Replicon chromosome
Accession NZ_CP095514
Organism Escherichia coli strain GN02323

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag MW403_RS18920 Protein ID WP_000244781.1
Coordinates 3981723..3982130 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag MW403_RS18925 Protein ID WP_000354046.1
Coordinates 3982111..3982377 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MW403_RS18900 (3977680) 3977680..3979413 - 1734 WP_000813189.1 single-stranded-DNA-specific exonuclease RecJ -
MW403_RS18905 (3979419) 3979419..3980129 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
MW403_RS18910 (3980154) 3980154..3981050 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
MW403_RS18915 (3981162) 3981162..3981683 + 522 WP_001055874.1 flavodoxin FldB -
MW403_RS18920 (3981723) 3981723..3982130 - 408 WP_000244781.1 protein YgfX Toxin
MW403_RS18925 (3982111) 3982111..3982377 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
MW403_RS18930 (3982620) 3982620..3983600 + 981 WP_000886079.1 tRNA-modifying protein YgfZ -
MW403_RS18935 (3983677) 3983677..3984336 - 660 WP_000250274.1 hemolysin III family protein -
MW403_RS18940 (3984500) 3984500..3984811 - 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
MW403_RS18945 (3984856) 3984856..3986289 + 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T242157 WP_000244781.1 NZ_CP095514:c3982130-3981723 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T242157 NZ_OW052188:c1113293-1112991 [Mycobacterium tuberculosis]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT242157 WP_000354046.1 NZ_CP095514:c3982377-3982111 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT242157 NZ_OW052188:c1113517-1113290 [Mycobacterium tuberculosis]
ATGACGAAAGAGAAGATCTCCGTGACGGTGGACGCGGCCGTCCTCGCGGCGATCGACGCGGACGCCAGGGCGGCGGGTTT
GAATCGGTCGGAAATGATTGAGCAGGCACTGCGCAACGAGCACCTGCGTGTCGCTCTGCGCGATTACACGGCTAAAACCG
TACCGGCGTTGGACATCGATGCCTACGCACAGCGGGTGTACCAGGCGAACCGGGCGGCCGGAAGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References