Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 2467239..2468827 Replicon chromosome
Accession NZ_CP095514
Organism Escherichia coli strain GN02323

Toxin (Protein)


Gene name hipA Uniprot ID A0A1L1KDK2
Locus tag MW403_RS11920 Protein ID WP_001125431.1
Coordinates 2467239..2468561 (-) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID V0VAH2
Locus tag MW403_RS11925 Protein ID WP_001304368.1
Coordinates 2468561..2468827 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MW403_RS11880 (2462878) 2462878..2463111 - 234 WP_000473544.1 hypothetical protein -
MW403_RS11885 (2463146) 2463146..2463397 - 252 WP_000381239.1 membrane protein -
MW403_RS11890 (2463400) 2463400..2463717 - 318 WP_000073835.1 DNA-binding protein -
MW403_RS11895 (2463729) 2463729..2464781 - 1053 WP_000568107.1 phage/plasmid replication protein, II/X family -
MW403_RS11900 (2464953) 2464953..2465399 + 447 WP_000527682.1 helix-turn-helix transcriptional regulator -
MW403_RS11905 (2465499) 2465499..2465921 + 423 WP_001527022.1 hypothetical protein -
MW403_RS11910 (2465989) 2465989..2466783 + 795 WP_001527023.1 hypothetical protein -
MW403_RS11915 (2466794) 2466794..2467060 + 267 WP_001527024.1 hypothetical protein -
MW403_RS11920 (2467239) 2467239..2468561 - 1323 WP_001125431.1 type II toxin-antitoxin system serine/threonine protein kinase toxin HipA Toxin
MW403_RS11925 (2468561) 2468561..2468827 - 267 WP_001304368.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
MW403_RS11930 (2469135) 2469135..2469893 + 759 WP_001286540.1 trans-aconitate 2-methyltransferase -
MW403_RS11935 (2469897) 2469897..2470811 - 915 WP_001443133.1 bestrophin family protein -
MW403_RS11940 (2471008) 2471008..2472459 - 1452 WP_000854660.1 tagaturonate reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - fimA 2417483..2468827 51344


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(4-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(14-69)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 49310.02 Da        Isoelectric Point: 8.6972

>T242145 WP_001125431.1 NZ_CP095514:c2468561-2467239 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVKRFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIAQIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKNSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Download         Length: 1323 bp

>T242145 NZ_OW052188:722327-722710 [Mycobacterium tuberculosis]
TTGGCGGCTGCAACGACAACCGGAACCCACCGAGGCCTGGAACTCCGCGCTGCTCAGCGGGCCGTCGGGTCGTGCGAACC
GCAACGAGCCGAGTTCTGCCGATCAGCGCGGAATGCGGACGAGTTCGACCAGATGAGCCGGATGTTTGGTGACGTCTACC
CCGATGTGCCAGTGCCGAAATCCGTGTGGCGGTGGATCGATTCGGCACAGCACCGCCTCGCCCGGGCGGGAGCGGTGGGT
GCCCTGTCGGTTGTCGATCTGCTGATCTGCGACACTGCGGCGGCCAGGGGCCTAGTAGTCCTCCACGACGATGCCGACTA
CGAACTCGCAGAGCGTCACCTTCCCGACATCAGAGTGCGGAGGGTCGTCAGCGCCGACGACTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9957.31 Da        Isoelectric Point: 4.9632

>AT242145 WP_001304368.1 NZ_CP095514:c2468827-2468561 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTSLTTFFKILQSLELSMTLCDAKNASPEA
AEQQDLEW

Download         Length: 267 bp

>AT242145 NZ_OW052188:722077-722232 [Mycobacterium tuberculosis]
GTGTCGGTGACCCAGATCGACCTCGACGACGAAGCGCTGGCCGATGTGATGCGCATCGCCGCCGTGCACACAAAGAAGGA
AGCCGTGAACCTCGCGATGCGCGATTACGTCGAACGGTTTCGGCGCATCGAGGCCCTGGCCCGCTCGCGCGAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1L1KDK2

Antitoxin

Source ID Structure
AlphaFold DB V0VAH2

References