Detailed information of TA system TA242141

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 922344..922894
• Replicon: chromosome
• Accession: NZ_CP095514
• Organism: Escherichia coli strain GN02323

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: S1PDG4
• Locus tag: MW403_RS04380
• Protein ID: WP_001160966.1
• Coordinates: 922580..922894 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V0VEX8
• Locus tag: MW403_RS04375
• Protein ID: WP_000125566.1
• Coordinates: 922344..922577 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MW403_RS04350 (917417)	917417..917704	+	288	WP_000203741.1	ferredoxin-like protein FixX	-
MW403_RS04355 (917763)	917763..919094	+	1332	WP_001183207.1	MFS transporter	-
MW403_RS04360 (919202)	919202..919732	+	531	WP_000600725.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
MW403_RS04365 (919725)	919725..921587	+	1863	WP_000377123.1	glutathione-regulated potassium-efflux system protein KefC	-
MW403_RS04370 (921779)	921779..922258	+	480	WP_000624375.1	type 3 dihydrofolate reductase	-
MW403_RS04375 (922344)	922344..922577	+	234	WP_000125566.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
MW403_RS04380 (922580)	922580..922894	+	315	WP_001160966.1	CcdB family protein	Toxin
MW403_RS04385 (922891)	922891..923739	-	849	WP_000257182.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
MW403_RS04390 (923746)	923746..924123	-	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
MW403_RS04395 (924126)	924126..924947	-	822	WP_001065363.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
MW403_RS04400 (924944)	924944..925933	-	990	WP_000241229.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
MW403_RS04405 (925933)	925933..927219	-	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11711.72 Da
• Isoelectric Point: 9.0851

>T242141 WP_001160966.1 NZ_CP095514:922580-922894 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Nucleotide

• Length: 315 bp

>T242141 NZ_OW052188:c634051-633623 [Mycobacterium tuberculosis]
ATGTTCCTTCTCGACGCCAACGTGCTGCTGGCTGCACACCGCGGTGACCACCCGAATCACCGAACCGTCCGCCCCTGGTT
CGATCGACTGCTCGCGGCTGACGACCCCTTCACAGTGCCGAACCTGGTATGGGCGTCGTTCCTCCGGCTGGCAACGAATC
GACGCATCTTCGAGATTCCGTCACCGCGAGCAGAGGCATTCGCATTCGTCGAAGCCGTCACCGCCCAGCCCCATCACCTT
CCGACGAACCCCGGTCCCAGACACCTGGTGCTGCTGCGAAAACTCTGCGACGAGGCCGACGCATCGGGCGACTTGATACC
TGACGCGGTACTCGCGGCCATAGCAGTGGGGCATCACTGCGCCGTGGTGAGCCTGGACAGGGATTTCGCCCGGTTTGCCT
CGGTGCGCCACATTCGCCCGCCGCTCTAG

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8530.53 Da
• Isoelectric Point: 5.8062

>AT242141 WP_000125566.1 NZ_CP095514:922344-922577 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT242141 NZ_OW052188:c634332-634075 [Mycobacterium tuberculosis]
ATGCGCACCACGGTGTCAATCTCCGATGAAATACTCGCTGCCGCCAAACGCCGGGCCCGCGAGCGTGGTCAATCGCTGGG
CGCTGTGATCGAGGACGCCCTTCGGCGGGAGTTCGCCGCCGCCCACGTCGGCGGCGCCCGCCCGACCGTCCCGGTTTTCG
ACGGCGGCACCGGTCCGCGGCGAGGCATCGACCTGACCTCGAATAGAGCGTTGTCCGAAGTGCTCGACGAGGGCCTGGAA
CTGAACTCCCGGAAGTAA

Structures

Toxin

• AlphaFold DB: A0A829G815

Antitoxin

• AlphaFold DB: A0A7U9LTM9