242129

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 30277..30802 Replicon plasmid p2270-1
Accession NZ_CP095511
Organism Escherichia coli strain GN02270

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag MW401_RS25265 Protein ID WP_001159871.1
Coordinates 30497..30802 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag MW401_RS25260 Protein ID WP_000813634.1
Coordinates 30277..30495 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MW401_RS25255 (27314) 27314..27955 - 642 WP_000990667.1 hypothetical protein -
MW401_RS25260 (30277) 30277..30495 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
MW401_RS25265 (30497) 30497..30802 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
MW401_RS25270 (30803) 30803..31612 + 810 WP_000016979.1 site-specific integrase -
MW401_RS25275 (31750) 31750..32025 - 276 WP_000239529.1 hypothetical protein -
MW401_RS25280 (32019) 32019..32663 - 645 WP_000633911.1 AAA family ATPase -
MW401_RS25285 (32892) 32892..33863 + 972 WP_001103694.1 plasmid segregation protein ParM -
MW401_RS25290 (33868) 33868..34260 + 393 WP_000340835.1 plasmid partitioning/stability family protein -
MW401_RS25295 (34265) 34265..34516 - 252 Protein_41 DUF4113 domain-containing protein -
MW401_RS25300 (34578) 34578..35375 - 798 WP_000544830.1 IS21-like element IS21 family helper ATPase IstB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B - 1..164623 164623


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T242129 WP_001159871.1 NZ_CP095511:30497-30802 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T242129 NZ_OW011623:31814-32092 [Thauera sp. PIV-1]
ATGCCGACTGAGCACAGACTGTTGCAACTCACCTGGACCCTCGCCGCTTGGGAAGACTACGAATACTGGCAGGGGCAGGA
CCGGAAGACGCTGAAGCGCATCAACACGCTCATTAAGGACTGCCTGCGCCACCCCTTTGAAGGCATCGGCAAGCCGGAGC
CGCTCAGGGAGAATCTCTCGGGCTTCTGGTCGCGCCGCATCGACGACGCCCATCGCCTCGTGTACTGCGCTGAAGGCGAG
TCGCTGATCATCATCGCCTGCCGTTACCACTACCAGTAG

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT242129 WP_000813634.1 NZ_CP095511:30277-30495 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT242129 NZ_OW011623:31567-31824 [Thauera sp. PIV-1]
ATGCGTGTAGTGAACTTCTCCGAGGCGCGCAATGGCCTCAAACACGTCATCGATCAGGTCGTCGACGATGCGGACTACAC
CGTCATCGCCCGCCGCGATGCGCCCGATGCCGTGCTGATGTCGCTCGACACCTTCAACAGCCTCATGGAAACCGTCCACC
TGTTGAAGTCCCCGGCCAACGCCGCACACCTGGCCCGCTCGATCGAACAATACCGCCAAGGCAACACGGCGCAGCGGGAG
CTGCGCGATGCCGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CQY2

References