Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4917160..4917657 Replicon chromosome
Accession NZ_CP095510
Organism Escherichia coli strain GN02270

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag MW401_RS23850 Protein ID WP_001173343.1
Coordinates 4917160..4917333 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag MW401_RS23855 Protein ID WP_000398619.1
Coordinates 4917361..4917657 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MW401_RS23840 (4913831) 4913831..4915288 + 1458 WP_000856848.1 dipeptide/tripeptide permease DtpC -
MW401_RS23845 (4915525) 4915525..4917042 + 1518 WP_000003806.1 lysine--tRNA ligase -
MW401_RS23850 (4917160) 4917160..4917333 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
MW401_RS23855 (4917361) 4917361..4917657 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
MW401_RS23860 (4917886) 4917886..4918158 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
MW401_RS23865 (4918170) 4918170..4918400 - 231 WP_000373291.1 4Fe-4S mono-cluster protein YjdI -
MW401_RS23870 (4918581) 4918581..4920212 + 1632 WP_001216477.1 sensor histidine kinase -
MW401_RS23875 (4920209) 4920209..4920928 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4908307..4917657 9350


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T242128 WP_001173343.1 NZ_CP095510:c4917333-4917160 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T242128 NZ_OW011623:31567-31824 [Thauera sp. PIV-1]
ATGCGTGTAGTGAACTTCTCCGAGGCGCGCAATGGCCTCAAACACGTCATCGATCAGGTCGTCGACGATGCGGACTACAC
CGTCATCGCCCGCCGCGATGCGCCCGATGCCGTGCTGATGTCGCTCGACACCTTCAACAGCCTCATGGAAACCGTCCACC
TGTTGAAGTCCCCGGCCAACGCCGCACACCTGGCCCGCTCGATCGAACAATACCGCCAAGGCAACACGGCGCAGCGGGAG
CTGCGCGATGCCGACTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT242128 WP_000398619.1 NZ_CP095510:c4917657-4917361 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT242128 NZ_OW011623:31814-32092 [Thauera sp. PIV-1]
ATGCCGACTGAGCACAGACTGTTGCAACTCACCTGGACCCTCGCCGCTTGGGAAGACTACGAATACTGGCAGGGGCAGGA
CCGGAAGACGCTGAAGCGCATCAACACGCTCATTAAGGACTGCCTGCGCCACCCCTTTGAAGGCATCGGCAAGCCGGAGC
CGCTCAGGGAGAATCTCTCGGGCTTCTGGTCGCGCCGCATCGACGACGCCCATCGCCTCGTGTACTGCGCTGAAGGCGAG
TCGCTGATCATCATCGCCTGCCGTTACCACTACCAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References