Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4035273..4035891 Replicon chromosome
Accession NZ_CP095510
Organism Escherichia coli strain GN02270

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag MW401_RS19630 Protein ID WP_001291435.1
Coordinates 4035673..4035891 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag MW401_RS19625 Protein ID WP_000344800.1
Coordinates 4035273..4035647 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MW401_RS19615 (4030362) 4030362..4031555 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
MW401_RS19620 (4031578) 4031578..4034727 + 3150 WP_001132480.1 efflux RND transporter permease AcrB -
MW401_RS19625 (4035273) 4035273..4035647 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
MW401_RS19630 (4035673) 4035673..4035891 + 219 WP_001291435.1 HHA domain-containing protein Toxin
MW401_RS19635 (4036063) 4036063..4036614 + 552 WP_000102559.1 maltose O-acetyltransferase -
MW401_RS19640 (4036730) 4036730..4037200 + 471 WP_000136192.1 YlaC family protein -
MW401_RS19645 (4037364) 4037364..4038914 + 1551 WP_001389864.1 cyclic-guanylate-specific phosphodiesterase PdeB -
MW401_RS19650 (4038956) 4038956..4039309 - 354 WP_000878140.1 DUF1428 domain-containing protein -
MW401_RS19660 (4039688) 4039688..4039999 + 312 WP_000409911.1 MGMT family protein -
MW401_RS19665 (4040030) 4040030..4040602 - 573 WP_000779826.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T242119 WP_001291435.1 NZ_CP095510:4035673-4035891 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T242119 NZ_OV754010:c5222717-5222334 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT242119 WP_000344800.1 NZ_CP095510:4035273-4035647 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT242119 NZ_OV754010:c5222959-5222717 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References