Detailed information of TA system TA242117

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3307228..3308012
• Replicon: chromosome
• Accession: NZ_CP095510
• Organism: Escherichia coli strain GN02270

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: V0T0H9
• Locus tag: MW401_RS16080
• Protein ID: WP_000613626.1
• Coordinates: 3307518..3308012 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: L4JCW6
• Locus tag: MW401_RS16075
• Protein ID: WP_001110447.1
• Coordinates: 3307228..3307521 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MW401_RS16065 (3302378)	3302378..3303337	-	960	WP_000846342.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-
MW401_RS16070 (3303910)	3303910..3307095	+	3186	WP_000827427.1	ribonuclease E	-
MW401_RS16075 (3307228)	3307228..3307521	+	294	WP_001110447.1	DUF1778 domain-containing protein	Antitoxin
MW401_RS16080 (3307518)	3307518..3308012	+	495	WP_000613626.1	GNAT family N-acetyltransferase	Toxin
MW401_RS16085 (3308107)	3308107..3309060	-	954	WP_001212768.1	flagellar hook-associated protein FlgL	-
MW401_RS16090 (3309072)	3309072..3310715	-	1644	WP_000096524.1	flagellar hook-associated protein FlgK	-
MW401_RS16095 (3310781)	3310781..3311722	-	942	WP_001309406.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
MW401_RS16100 (3311722)	3311722..3312819	-	1098	WP_000589320.1	flagellar basal body P-ring protein FlgI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (81-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18084.00 Da
• Isoelectric Point: 8.2617

>T242117 WP_000613626.1 NZ_CP095510:3307518-3308012 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T242117 NZ_OV754010:c4741222-4740938 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCTGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11109.69 Da
• Isoelectric Point: 10.5423

>AT242117 WP_001110447.1 NZ_CP095510:3307228-3307521 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT242117 NZ_OV754010:c4741454-4741212 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: V0T0H9

Antitoxin

• AlphaFold DB: L4JCW6