Detailed information of TA system TA242116

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2910949..2911587
• Replicon: chromosome
• Accession: NZ_CP095510
• Organism: Escherichia coli strain GN02270

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: B7N4J4
• Locus tag: MW401_RS13925
• Protein ID: WP_000813797.1
• Coordinates: 2911411..2911587 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: MW401_RS13920
• Protein ID: WP_076611057.1
• Coordinates: 2910949..2911365 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MW401_RS13900 (2906101)	2906101..2907042	-	942	WP_001251335.1	ABC transporter permease	-
MW401_RS13905 (2907043)	2907043..2908056	-	1014	WP_000220413.1	ABC transporter ATP-binding protein	-
MW401_RS13910 (2908074)	2908074..2909219	-	1146	WP_000047447.1	ABC transporter substrate-binding protein	-
MW401_RS13915 (2909464)	2909464..2910870	-	1407	WP_000760613.1	PLP-dependent aminotransferase family protein	-
MW401_RS13920 (2910949)	2910949..2911365	-	417	WP_076611057.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
MW401_RS13925 (2911411)	2911411..2911587	-	177	WP_000813797.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
MW401_RS13930 (2911809)	2911809..2912039	+	231	WP_000494244.1	YncJ family protein	-
MW401_RS13935 (2912131)	2912131..2914092	-	1962	WP_024189226.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
MW401_RS13940 (2914165)	2914165..2914701	-	537	WP_000429161.1	DNA-binding transcriptional regulator SutR	-
MW401_RS13945 (2914754)	2914754..2915965	+	1212	WP_001323162.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6724.76 Da
• Isoelectric Point: 11.5666

>T242116 WP_000813797.1 NZ_CP095510:c2911587-2911411 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFQGRRSVMPRHPSDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T242116 NZ_OV754010:c4661190-4660849 [Klebsiella pneumoniae]
ATGAAAACCTTACCTGCAACAATTCAGCGGGCGGCTAAGCCGTGCCCGTCTCCCGTGACGGTCTGGCAGATGCTGCTTAC
CCGTCTGCTGGAGCAACATTATGGCCTTACCCTGAATGACACGCCGTTCAGCGATGAAACCGTTATTCAGGAACATATCA
ACGCAGGCATCACACTGGTTGATGCCGTTAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCGCAGAGGGTTT
AACTGTCAGGAACAGTCACCTTATCTGGGCGCAGTGGATATTTTGCGGGCGCGGCAGGCAACCGGCCTGTTACGGCAAAG
ACATCTTCCTTCGATACGCTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15251.65 Da
• Isoelectric Point: 4.5908

>AT242116 WP_076611057.1 NZ_CP095510:c2911365-2910949 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLTNNDHFIEVPLSIASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT242116 NZ_OV754010:c4661540-4661214 [Klebsiella pneumoniae]
ATGCCACTGACTGATGCCATACAGTGGGGATTAAAGCGAAGTTTCACACCGAATTTTGGCGCAAGGCTGGTACAGGAAGG
CAACCGACTGCACTACCTGGCTGACCGGGCCAATATCACCGGTAAATTTGGTGACGCCGAATGTCTGAAGCTGGAAGAAG
CATTCCCGCATTTTATCCGCCAGATGGAACTGATGCTGAGCACTGGCGAACTTAATCCCCGTCATGCGCACAGCGTCACT
CTCTACCACAACGCTTTCACCTGTGAAGCCGATACTCTCGGTAGTTGTGGTTACGTCTACATCGTTATTTATCCCACTCA
ACGTTAA

Structures

Toxin

• AlphaFold DB: A0A829J715