Detailed information of TA system TA242114

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2810015..2811603
• Replicon: chromosome
• Accession: NZ_CP095510
• Organism: Escherichia coli strain GN02270

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: U9YVT8
• Locus tag: MW401_RS13470
• Protein ID: WP_001125415.1
• Coordinates: 2810281..2811603 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: MW401_RS13465
• Protein ID: WP_001296726.1
• Coordinates: 2810015..2810281 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MW401_RS13465 (2810015)	2810015..2810281	+	267	WP_001296726.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
MW401_RS13470 (2810281)	2810281..2811603	+	1323	WP_001125415.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
MW401_RS13475 (2812001)	2812001..2812900	-	900	WP_001057776.1	LysR family transcriptional regulator	-
MW401_RS13480 (2813040)	2813040..2813348	+	309	WP_001221046.1	hypothetical protein	-
MW401_RS13485 (2813357)	2813357..2814046	+	690	WP_000801313.1	type 1 glutamine amidotransferase domain-containing protein	-
MW401_RS13490 (2814120)	2814120..2815067	+	948	WP_000854796.1	aldo/keto reductase	-
MW401_RS13495 (2815170)	2815170..2815325	+	156	WP_001322415.1	hypothetical protein	-
MW401_RS13500 (2815285)	2815285..2815704	+	420	WP_001322416.1	amidohydrolase family protein	-
MW401_RS13505 (2815694)	2815694..2815846	+	153	WP_001322417.1	amidohydrolase family protein	-
MW401_RS13510 (2815997)	2815997..2816569	+	573	WP_000789913.1	NAD(P)H-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49384.05 Da
• Isoelectric Point: 8.5830

>T242114 WP_001125415.1 NZ_CP095510:2810281-2811603 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLANRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGAGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHRRLSREYGIK

Nucleotide

• Length: 1323 bp

>T242114 NZ_OV754010:4002048-4002266 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10047.39 Da
• Isoelectric Point: 4.9632

>AT242114 WP_001296726.1 NZ_CP095510:2810015-2810281 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT242114 NZ_OV754010:4001647-4002021 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: U9YVT8