Detailed information of TA system TA024211

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 5335171..5335766
• Replicon: chromosome
• Accession: NC_013008
• Organism: Escherichia coli O157:H7 str. TW14359

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: U9Y4M4
• Locus tag: ECSP_RS27670
• Protein ID: WP_000239579.1
• Coordinates: 5335416..5335766 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: Q8XCF3
• Locus tag: ECSP_RS27665
• Protein ID: WP_001223210.1
• Coordinates: 5335171..5335422 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECSP_RS27655	5330836..5334615	+	3780	WP_000060930.1	autotransporter assembly complex protein TamB	-
ECSP_RS27660	5334618..5334959	+	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
ECSP_RS27665	5335171..5335422	+	252	WP_001223210.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
ECSP_RS27670	5335416..5335766	+	351	WP_000239579.1	endoribonuclease toxin ChpB	Toxin
ECSP_RS27675	5335846..5336376	-	531	WP_000055075.1	inorganic diphosphatase	-
ECSP_RS27680	5336686..5337642	+	957	WP_000265942.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
ECSP_RS33830	5337782..5339284	+	1503	Protein_5205	sugar ABC transporter ATP-binding protein	-
ECSP_RS27690	5339298..5340320	+	1023	WP_001301928.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12511.45 Da
• Isoelectric Point: 5.6219

>T24211 WP_000239579.1 NC_013008:5335416-5335766 [Escherichia coli O157:H7 str. TW14359]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T24211 NC_013008:5335416-5335766 [Escherichia coli O157:H7 str. TW14359]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GTGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTT
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9299.64 Da
• Isoelectric Point: 4.4201

>AT24211 WP_001223210.1 NC_013008:5335171-5335422 [Escherichia coli O157:H7 str. TW14359]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEVQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT24211 NC_013008:5335171-5335422 [Escherichia coli O157:H7 str. TW14359]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGTGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y8A8