Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2255547..2256378 Replicon chromosome
Accession NZ_CP095510
Organism Escherichia coli strain GN02270

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag MW401_RS10680 Protein ID WP_000854814.1
Coordinates 2255547..2255921 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID B3Y195
Locus tag MW401_RS10685 Protein ID WP_001285585.1
Coordinates 2256010..2256378 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MW401_RS10640 (2250943) 2250943..2252109 + 1167 WP_000830156.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
MW401_RS10645 (2252228) 2252228..2252701 + 474 WP_001105415.1 DNA gyrase inhibitor SbmC -
MW401_RS10650 (2252899) 2252899..2253957 + 1059 WP_001200891.1 FUSC family protein -
MW401_RS10655 (2254129) 2254129..2254458 + 330 WP_000450409.1 DUF496 family protein -
MW401_RS10660 (2254559) 2254559..2254882 - 324 WP_225343796.1 EutP/PduV family microcompartment system protein -
MW401_RS10665 (2254861) 2254861..2254941 + 81 WP_023441679.1 hypothetical protein -
MW401_RS10670 (2255230) 2255230..2255310 - 81 Protein_2080 hypothetical protein -
MW401_RS10675 (2255356) 2255356..2255550 - 195 WP_000988600.1 DUF5983 family protein -
MW401_RS10680 (2255547) 2255547..2255921 - 375 WP_000854814.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
MW401_RS10685 (2256010) 2256010..2256378 - 369 WP_001285585.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
MW401_RS10690 (2256452) 2256452..2256673 - 222 WP_000692323.1 DUF987 domain-containing protein -
MW401_RS10695 (2256736) 2256736..2257212 - 477 WP_001186724.1 RadC family protein -
MW401_RS10700 (2257228) 2257228..2257713 - 486 WP_001469549.1 antirestriction protein -
MW401_RS10705 (2257768) 2257768..2258586 - 819 WP_001234620.1 DUF932 domain-containing protein -
MW401_RS10710 (2258686) 2258686..2258919 - 234 WP_001119729.1 DUF905 family protein -
MW401_RS10715 (2258998) 2258998..2259453 - 456 WP_000581504.1 IrmA family protein -
MW401_RS10720 (2259529) 2259529..2260110 - 582 Protein_2090 hypothetical protein -
MW401_RS10725 (2260167) 2260167..2260961 - 795 WP_000544809.1 IS21-like element helper ATPase IstB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T242105 WP_000854814.1 NZ_CP095510:c2255921-2255547 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T242105 NZ_OV753987:c4741112-4740828 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCTGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13593.48 Da        Isoelectric Point: 6.3139

>AT242105 WP_001285585.1 NZ_CP095510:c2256378-2256010 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT242105 NZ_OV753987:c4741344-4741102 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LW60

References