Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1250439..1251093 Replicon chromosome
Accession NZ_CP095510
Organism Escherichia coli strain GN02270

Toxin (Protein)


Gene name cptA Uniprot ID B7N7E0
Locus tag MW401_RS06085 Protein ID WP_000244769.1
Coordinates 1250686..1251093 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag MW401_RS06080 Protein ID WP_000354046.1
Coordinates 1250439..1250705 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MW401_RS06060 (1246527) 1246527..1247960 - 1434 WP_001513393.1 6-phospho-beta-glucosidase BglA -
MW401_RS06065 (1248005) 1248005..1248316 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
MW401_RS06070 (1248480) 1248480..1249139 + 660 WP_000250274.1 hemolysin III family protein -
MW401_RS06075 (1249216) 1249216..1250196 - 981 WP_000886049.1 tRNA-modifying protein YgfZ -
MW401_RS06080 (1250439) 1250439..1250705 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
MW401_RS06085 (1250686) 1250686..1251093 + 408 WP_000244769.1 protein YgfX Toxin
MW401_RS06090 (1251133) 1251133..1251654 - 522 WP_001055874.1 flavodoxin FldB -
MW401_RS06095 (1251766) 1251766..1252662 + 897 WP_000806652.1 site-specific tyrosine recombinase XerD -
MW401_RS06100 (1252687) 1252687..1253397 + 711 WP_000715210.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
MW401_RS06105 (1253403) 1253403..1255136 + 1734 WP_000813190.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16062.03 Da        Isoelectric Point: 11.5554

>T242103 WP_000244769.1 NZ_CP095510:1250686-1251093 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGK
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T242103 NZ_OV753987:c4617356-4616823 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTGATGCGAGTGGTTTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT242103 WP_000354046.1 NZ_CP095510:1250439-1250705 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT242103 NZ_OV753987:c4617633-4617367 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829J3T1

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References