Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 3638911..3639461 Replicon chromosome
Accession NZ_CP095505
Organism Escherichia coli strain GN02050

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag MW398_RS18795 Protein ID WP_001160966.1
Coordinates 3639147..3639461 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag MW398_RS18790 Protein ID WP_000125566.1
Coordinates 3638911..3639144 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MW398_RS18765 (3633984) 3633984..3634271 + 288 WP_000203741.1 ferredoxin-like protein FixX -
MW398_RS18770 (3634330) 3634330..3635661 + 1332 WP_001183207.1 MFS transporter -
MW398_RS18775 (3635769) 3635769..3636299 + 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
MW398_RS18780 (3636292) 3636292..3638154 + 1863 WP_021523578.1 glutathione-regulated potassium-efflux system protein KefC -
MW398_RS18785 (3638346) 3638346..3638825 + 480 WP_059342386.1 type 3 dihydrofolate reductase -
MW398_RS18790 (3638911) 3638911..3639144 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
MW398_RS18795 (3639147) 3639147..3639461 + 315 WP_001160966.1 CcdB family protein Toxin
MW398_RS18800 (3639458) 3639458..3640306 - 849 WP_001765384.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
MW398_RS18805 (3640313) 3640313..3640690 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
MW398_RS18810 (3640693) 3640693..3641514 - 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
MW398_RS18815 (3641511) 3641511..3642500 - 990 WP_000241229.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
MW398_RS18820 (3642500) 3642500..3643786 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T242086 WP_001160966.1 NZ_CP095505:3639147-3639461 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T242086 NZ_OV648025:c2358243-2357977 [Staphylococcus argenteus]
ATGGCTAGGTTAAACATTACGTTCTCGCCTCAGGCATTTGAAGATTATGAGTATTTTCAGCAGAACGATAAAAAAATGGT
AAAGAAGATTAATGAGTTACTTAAAAGCATTGATAGAAATGGTGCATTGCAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATTTATTTGGCTATTATAGTAGACGTATCAATCACGAACATAGGTTGGTTTATACAGTTGACGATAATCACATAAAA
ATAGCATCCTGTAGATACCATTATTAG

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT242086 WP_000125566.1 NZ_CP095505:3638911-3639144 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT242086 NZ_OV648025:c2358494-2358243 [Staphylococcus argenteus]
ATGATTGTAAAAAACTATTCTTACGCTAGACAGAATTTAAAGACGCTTATGACAAAGGTAAATGACGATAGTGATGTGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATTATGTCAGAAGCAGATTATAATTCTATAATTGAAACCCTTTACC
TTCAACAGAATCCAAATAATGCTGAACACTTAGCCCAATCAATTGCTGACTTAGAACGAGGCAAAATGGTAACGAAAGAA
ATAGATGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References