Detailed information of TA system TA242085

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 3532223..3532799
• Replicon: chromosome
• Accession: NZ_CP095505
• Organism: Escherichia coli strain GN02050

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: D3GXZ2
• Locus tag: MW398_RS18265
• Protein ID: WP_001297643.1
• Coordinates: 3532223..3532510 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: MW398_RS18270
• Protein ID: WP_021538262.1
• Coordinates: 3532497..3532799 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MW398_RS18245 (3528476)	3528476..3529756	-	1281	WP_001037380.1	DUF445 domain-containing protein	-
MW398_RS18250 (3529797)	3529797..3531029	-	1233	WP_001339512.1	multidrug efflux MFS transporter MdtM	-
MW398_RS18255 (3531175)	3531175..3531270	-	96	Protein_3385	dienelactone hydrolase family protein	-
MW398_RS18260 (3531327)	3531327..3532024	+	698	Protein_3386	IS1 family transposase	-
MW398_RS18265 (3532223)	3532223..3532510	+	288	WP_001297643.1	BrnT family toxin	Toxin
MW398_RS18270 (3532497)	3532497..3532799	+	303	WP_021538262.1	BrnA antitoxin family protein	Antitoxin
MW398_RS18275 (3532833)	3532833..3533789	-	957	WP_001297640.1	GTPase	-
MW398_RS18280 (3533800)	3533800..3534003	-	204	WP_000467859.1	YbdD/YjiX family protein	-
MW398_RS18285 (3534053)	3534053..3536203	-	2151	WP_001295524.1	pyruvate/proton symporter BtsT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11295.77 Da
• Isoelectric Point: 6.8821

>T242085 WP_001297643.1 NZ_CP095505:3532223-3532510 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Nucleotide

• Length: 288 bp

>T242085 NZ_OV648025:c2042022-2041813 [Staphylococcus argenteus]
ATGGTACATCAAAACACAATATATACTGCAGGTATTGAAACTGAAGAACAAGTAAACCAGTTGACAGAACGTATATCAAA
CATGATAGGTGTGCATAAAGTGAATATTAATATCATTGATGGAGAAGTAGTTGTAGCATATGAAACACCCGCAAATTTAA
ATAGCATTGAAAAAGAAATTTATGATACAGGCTATAAAATTGTATATTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11531.00 Da
• Isoelectric Point: 9.4651

>AT242085 WP_021538262.1 NZ_CP095505:3532497-3532799 [Escherichia coli]
MSMVKHKRGTSSTLDVHHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT242085 NZ_OV648025:c2042327-2042034 [Staphylococcus argenteus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATTAAAACAAATCTTAAATCACGTTTAAATCGAATAGAAGGTCA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTTCTTACACAAATAAGAGCGACGCGTTCTG
CATTAAACAGTGTCGCAATAAAGTTATTAGAGCAACATATGAAAAGTTGTATTATGAATAAAGTAAATCAAGGTGCTCAA
GAAGAAGCGATGGAAGAGTTACTAGTTACTTTTCAAAAGTTGATTAAAGATTAA

Structures

Toxin

• AlphaFold DB: A0A829GEV9