Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3149289..3149786 Replicon chromosome
Accession NZ_CP095505
Organism Escherichia coli strain GN02050

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag MW398_RS16330 Protein ID WP_001173343.1
Coordinates 3149613..3149786 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag MW398_RS16325 Protein ID WP_001312331.1
Coordinates 3149289..3149585 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MW398_RS16305 (3146019) 3146019..3146738 - 720 WP_000611282.1 two-component system response regulator DcuR -
MW398_RS16310 (3146735) 3146735..3148366 - 1632 WP_001216466.1 sensor histidine kinase -
MW398_RS16315 (3148547) 3148547..3148777 + 231 WP_000373291.1 4Fe-4S mono-cluster protein YjdI -
MW398_RS16320 (3148789) 3148789..3149061 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
MW398_RS16325 (3149289) 3149289..3149585 + 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
MW398_RS16330 (3149613) 3149613..3149786 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
MW398_RS16335 (3149905) 3149905..3151422 - 1518 WP_000003805.1 lysine--tRNA ligase -
MW398_RS16340 (3151659) 3151659..3153116 - 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T242081 WP_001173343.1 NZ_CP095505:3149613-3149786 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T242081 NZ_OV408205:22101-22517 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT242081 WP_001312331.1 NZ_CP095505:3149289-3149585 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT242081 NZ_OV408205:21874-22104 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References