Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1693218..1693872 Replicon chromosome
Accession NZ_CP095505
Organism Escherichia coli strain GN02050

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag MW398_RS09350 Protein ID WP_000244781.1
Coordinates 1693218..1693625 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag MW398_RS09355 Protein ID WP_000354046.1
Coordinates 1693606..1693872 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MW398_RS09330 (1689175) 1689175..1690908 - 1734 WP_000813189.1 single-stranded-DNA-specific exonuclease RecJ -
MW398_RS09335 (1690914) 1690914..1691624 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
MW398_RS09340 (1691649) 1691649..1692545 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
MW398_RS09345 (1692657) 1692657..1693178 + 522 WP_001055874.1 flavodoxin FldB -
MW398_RS09350 (1693218) 1693218..1693625 - 408 WP_000244781.1 protein YgfX Toxin
MW398_RS09355 (1693606) 1693606..1693872 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
MW398_RS09360 (1694115) 1694115..1695095 + 981 WP_000886080.1 tRNA-modifying protein YgfZ -
MW398_RS09365 (1695172) 1695172..1695831 - 660 WP_000250274.1 hemolysin III family protein -
MW398_RS09370 (1695995) 1695995..1696306 - 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
MW398_RS09375 (1696351) 1696351..1697784 + 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T242079 WP_000244781.1 NZ_CP095505:c1693625-1693218 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T242079 NZ_OV408202:c5306427-5306044 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT242079 WP_000354046.1 NZ_CP095505:c1693872-1693606 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT242079 NZ_OV408202:c5306669-5306427 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References