Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4418570..4419201 Replicon chromosome
Accession NC_013008
Organism Escherichia coli O157:H7 str. TW14359

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag ECSP_RS23180 Protein ID WP_001259385.1
Coordinates 4418926..4419201 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag ECSP_RS23175 Protein ID WP_000593555.1
Coordinates 4418570..4418929 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECSP_RS23145 4413682..4414155 + 474 WP_001161553.1 PTS sugar transporter subunit IIA -
ECSP_RS23150 4414152..4414433 + 282 WP_000084025.1 PTS sugar transporter subunit IIB -
ECSP_RS23155 4414510..4415868 + 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
ECSP_RS23160 4415861..4417369 + 1509 WP_001301773.1 carbohydrate kinase -
ECSP_RS23165 4417359..4417628 + 270 WP_000933060.1 HPr family phosphocarrier protein -
ECSP_RS23170 4417660..4418520 + 861 WP_001131788.1 class II aldolase -
ECSP_RS23175 4418570..4418929 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
ECSP_RS23180 4418926..4419201 - 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
ECSP_RS23185 4419274..4420398 - 1125 WP_001301659.1 ABC transporter permease -
ECSP_RS23190 4420398..4423133 - 2736 WP_000149110.1 ribosome-associated ATPase/putative transporter RbbA -
ECSP_RS23195 4423130..4424197 - 1068 WP_000361458.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T24207 WP_001259385.1 NC_013008:c4419201-4418926 [Escherichia coli O157:H7 str. TW14359]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T24207 NC_013008:c4419201-4418926 [Escherichia coli O157:H7 str. TW14359]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT24207 WP_000593555.1 NC_013008:c4418929-4418570 [Escherichia coli O157:H7 str. TW14359]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT24207 NC_013008:c4418929-4418570 [Escherichia coli O157:H7 str. TW14359]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References