Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 47560..48085 Replicon plasmid p2050-1
Accession NZ_CP095504
Organism Escherichia coli strain GN02050

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag MW398_RS00505 Protein ID WP_001159871.1
Coordinates 47560..47865 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag MW398_RS00510 Protein ID WP_000813634.1
Coordinates 47867..48085 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MW398_RS00475 (42827) 42827..44097 + 1271 Protein_60 translesion error-prone DNA polymerase V subunit UmuC -
MW398_RS00480 (44102) 44102..44494 - 393 WP_000340835.1 plasmid partitioning/stability family protein -
MW398_RS00485 (44499) 44499..45470 - 972 WP_001103694.1 plasmid segregation protein ParM -
MW398_RS00490 (45699) 45699..46343 + 645 WP_000633911.1 AAA family ATPase -
MW398_RS00495 (46337) 46337..46612 + 276 WP_000239529.1 hypothetical protein -
MW398_RS00500 (46750) 46750..47559 - 810 WP_000016979.1 site-specific integrase -
MW398_RS00505 (47560) 47560..47865 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
MW398_RS00510 (47867) 47867..48085 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
MW398_RS00515 (50407) 50407..51048 + 642 WP_021537822.1 hypothetical protein -
MW398_RS00520 (51288) 51288..51668 + 381 WP_001171554.1 transposase -
MW398_RS00525 (51665) 51665..52012 + 348 WP_000612591.1 IS66 family insertion sequence element accessory protein TnpB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - senB 1..120616 120616


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T242065 WP_001159871.1 NZ_CP095504:c47865-47560 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T242065 NZ_OV350343:364683-364982 [Halomonas sp. TD01]
ATGCTCAGCTTCCGTATCACGCCAAGGGCAAGAGACGATTTAAAAAATATCGGCCGCTATACGGAGTGGCAATGGGGCAA
AAACCAACGAAATACCTATTTGAAGAATTTTGAAAAACGGTTTCACTGGCTCGCAGAAAATCCCCACCTGGGAAAGCACC
GCTCGGATGTTAGCGAGGGTTATTACAGCTACCCTCAGGGCCAGCACGTTATCTTTTATCTAATCGGCCAGGAATGCATT
GAAATCATTGGCATTCCTCACAAAGAGATGGATATCGTTTCATATTTTCTGCCTGAATGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT242065 WP_000813634.1 NZ_CP095504:c48085-47867 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT242065 NZ_OV350343:364435-364683 [Halomonas sp. TD01]
ATGGCTACGACCAGTCTTAGCTTAGGGGAGCACTGGGAAGTCTTTATCAGAAACGAGGTCGCCAGCGGACGCTACGGCTC
CGCAAGTGAAGTTGTGCGTGATGCGTTACGTGCAATGGAAGAGCGCAAAAGCAAACTTGAAGCGCTTAGAGCTCACTTAG
CCCAAGGCGCAGAGCAAGCGAGAGCAGGTGAATTCGTGGATGATTTTTCGATGGATGCATTAATCAACGATTTGGATAGT
GAAACGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References