Detailed information of TA system TA242061

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 1985730..1986403
• Replicon: chromosome
• Accession: NZ_CP095498
• Organism: Burkholderia cepacia strain BRDJ

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: A0A0J5X1U9
• Locus tag: MW307_RS31560
• Protein ID: WP_027787436.1
• Coordinates: 1985987..1986403 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: MW307_RS31555
• Protein ID: WP_060196597.1
• Coordinates: 1985730..1985990 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MW307_RS31540	1981308..1983560	+	2253	WP_119338655.1	TonB-dependent siderophore receptor	-
MW307_RS31545	1983624..1984463	+	840	WP_119338656.1	formyltransferase family protein	-
MW307_RS31550	1984463..1985479	+	1017	WP_027787437.1	GNAT family N-acetyltransferase	-
MW307_RS31555	1985730..1985990	+	261	WP_060196597.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MW307_RS31560	1985987..1986403	+	417	WP_027787436.1	PIN domain-containing protein	Toxin
MW307_RS31565	1986458..1987363	-	906	WP_119338657.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
MW307_RS31570	1987332..1988405	-	1074	WP_119338658.1	type IV toxin-antitoxin system AbiEi family antitoxin	-
MW307_RS31575	1988747..1989910	+	1164	WP_059700208.1	M20 aminoacylase family protein	-
MW307_RS31580	1990064..1990603	+	540	WP_027787432.1	outer membrane protein assembly factor BamE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-130)

Antitoxin

• SpoVT-AbrB domain (7-54)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 14536.66 Da
• Isoelectric Point: 7.8895

>T242061 WP_027787436.1 NZ_CP095498:1985987-1986403 [Burkholderia cepacia]
VTTLYMLDTNICSFIMRERPPVVLSRLQSCVNAQHRIVVSAITYAEMRFGAVGRKASPKHAELVTAFVSRLDGVLSWDTA
AVDATAAIRADLAARGTPIGANDASIAGHAIAAGAVLVTNNGREFGRVAGLSLEDWAA

Nucleotide

• Length: 417 bp

>T242061 NZ_OV100759:c173083-172901 [Aggregatibacter sp. Marseille-P9115]
GTGGATAGTCGAAAAATAATTAAGATGATTGAAGCAGATGGTTGGATTTTTCACCATGCCACAGGAAGCCACTATCACTT
TACTCATCCGATAAAGAAAGGGATTGTGACCGTTCCACATCCTAAAAAGGATTTAAAGAAAGGGACGGAAAATTCAATAT
TAAAACAAGCAAGGCTAAAATAG

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9592.89 Da
• Isoelectric Point: 4.6151

>AT242061 WP_060196597.1 NZ_CP095498:1985730-1985990 [Burkholderia cepacia]
MRTVSIFKNARNQAIRIPKDMEFEGVTELEIRREGDTLMLRPVRPTWLSFASEPLADADFLVERPAVIESGRFDLSGGAA
PTESGQ

Nucleotide

• Length: 261 bp

>AT242061 NZ_OV100759:c172871-172458 [Aggregatibacter sp. Marseille-P9115]
ATGTTATATCCAATCGCAATTGAGCCAGGTGACGAAACTCACGCATTTGGTGTGATCGTGCCGGATATTCCTGGCTGTTT
TTCTGCCGGTGATACGCTTGAAGAAGCTTACACCAATGCAAAAGCAGCCATTGCCGGGCATTTAGAATTATTGGTTGAAA
TGGGTGAAGAAGTGCCTTTACCAACATCAATGGAAAATCATCGTAACAACCCTGATTTTACTAACTACGGTATGTTTTTT
GGTGTGGTTGATGTGGATATTACGCATTTATTGGGTAAGTCTGAAAAAATCAACATCACAATGCCGGCTTATTTAATTAA
GCGTATTGATGATTTTGTTTCAACGCACCGTGAATATAAAAACCGTAGCAGTTTTTTAGCCAAAATCGCAGCAGATAAGA
TCTTATCTGCGTAA

Structures

Toxin

• AlphaFold DB: A0A0J5X1U9