Detailed information of TA system TA242055

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 1473667..1474244
• Replicon: chromosome
• Accession: NZ_CP095497
• Organism: Burkholderia cepacia strain BRDJ

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: MW307_RS13105
• Protein ID: WP_060358899.1
• Coordinates: 1473667..1474032 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U2HEK9
• Locus tag: MW307_RS13110
• Protein ID: WP_021160898.1
• Coordinates: 1474026..1474244 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MW307_RS13085	1469454..1470008	+	555	WP_027791286.1	carboxymuconolactone decarboxylase family protein	-
MW307_RS13090	1470169..1470945	+	777	WP_119336645.1	extensin	-
MW307_RS13095	1471040..1472638	-	1599	WP_059492406.1	alkyl hydroperoxide reductase subunit F	-
MW307_RS13100	1472767..1473330	-	564	WP_006488685.1	alkyl hydroperoxide reductase subunit C	-
MW307_RS13105	1473667..1474032	-	366	WP_060358899.1	type II toxin-antitoxin system VapC family toxin	Toxin
MW307_RS13110	1474026..1474244	-	219	WP_021160898.1	hypothetical protein	Antitoxin
MW307_RS13115	1474335..1475231	-	897	WP_048026027.1	LysR substrate-binding domain-containing protein	-
MW307_RS13120	1475409..1476575	+	1167	WP_119336644.1	branched-chain amino acid ABC transporter substrate-binding protein	-
MW307_RS13125	1476630..1477154	+	525	WP_021160901.1	L-2-amino-thiazoline-4-carboxylic acid hydrolase	-
MW307_RS13130	1477147..1478412	+	1266	WP_119336643.1	Zn-dependent hydrolase	-
MW307_RS13135	1478500..1478982	-	483	WP_119336642.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-106)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13390.42 Da
• Isoelectric Point: 8.4389

>T242055 WP_060358899.1 NZ_CP095497:c1474032-1473667 [Burkholderia cepacia]
MVKALFDTNILIDYLGGVESARAELGRYDYRAISTISWMEVLAGTSAQDEAPIRAWLSSFDVIALDSPVANRAVAIRKER
RIRLPDAIVWASAQVNGLLLVSRNTKDFPATEPGVRVPYKL

Nucleotide

• Length: 366 bp

>T242055 NZ_OV049808:3699569-3700051 [Klebsiella pneumoniae]
GTGGGACGTGTAACCGCGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTTGA
TGAATGGCTAAAGCACAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGGACGA
AGCAAGTAGCTGGTTTTTACTCTCTGGCCACCGGTAGCGTTAACCATACGGAAACTACAGGCAGACTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCCGTTCTACGCTGTTATCGCGTAGCCGAGAATATTGGCGTCCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCTAAAGGCTTCTATACTCACCATGGATTTACGGCATCGCAAACTCATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8372.51 Da
• Isoelectric Point: 5.0604

>AT242055 WP_021160898.1 NZ_CP095497:c1474244-1474026 [Burkholderia cepacia]
MSRILVDLSDGQLDELAVIVETQHRPRAAIIRDAIDAYIALHKHRLADDVFGLWKDRKVDGLTYQEELRSEW

Nucleotide

• Length: 219 bp

>AT242055 NZ_OV049808:3699315-3699581 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCTTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Antitoxin

• AlphaFold DB: U2HEK9