Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | hicAB/- |
| Location | 12722..13314 | Replicon | plasmid pLS45-4 |
| Accession | NZ_CP095452 | ||
| Organism | Escherichia coli strain LS45 | ||
Toxin (Protein)
| Gene name | hicA | Uniprot ID | A0A1I9ZJC5 |
| Locus tag | MWN28_RS26640 | Protein ID | WP_000513532.1 |
| Coordinates | 12722..12970 (+) | Length | 83 a.a. |
Antitoxin (Protein)
| Gene name | hicB | Uniprot ID | A0A1I9ZJ49 |
| Locus tag | MWN28_RS26645 | Protein ID | WP_000681609.1 |
| Coordinates | 12967..13314 (+) | Length | 116 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| MWN28_RS26610 (MWN28_26620) | 8062..8937 | - | 876 | WP_032492042.1 | extended-spectrum class A beta-lactamase CTX-M-64 | - |
| MWN28_RS26615 (MWN28_26625) | 9190..10452 | - | 1263 | WP_000608644.1 | IS1380-like element ISEcp1 family transposase | - |
| MWN28_RS26620 (MWN28_26630) | 10852..11070 | + | 219 | WP_154101533.1 | hypothetical protein | - |
| MWN28_RS26625 (MWN28_26635) | 11202..11489 | + | 288 | WP_000673430.1 | hypothetical protein | - |
| MWN28_RS26630 (MWN28_26640) | 11541..11915 | + | 375 | WP_015387341.1 | hypothetical protein | - |
| MWN28_RS26635 (MWN28_26645) | 11930..12223 | + | 294 | WP_000954637.1 | hypothetical protein | - |
| MWN28_RS26640 (MWN28_26650) | 12722..12970 | + | 249 | WP_000513532.1 | type II toxin-antitoxin system HicA family toxin | Toxin |
| MWN28_RS26645 (MWN28_26655) | 12967..13314 | + | 348 | WP_000681609.1 | type II toxin-antitoxin system HicB family antitoxin | Antitoxin |
| MWN28_RS26650 (MWN28_26660) | 13402..13845 | - | 444 | WP_023223351.1 | hypothetical protein | - |
| MWN28_RS26655 (MWN28_26665) | 14086..14385 | + | 300 | WP_034169410.1 | type II toxin-antitoxin system HicB family antitoxin | - |
| MWN28_RS26660 (MWN28_26670) | 14645..14971 | - | 327 | WP_000206697.1 | hypothetical protein | - |
| MWN28_RS26665 (MWN28_26675) | 14973..15296 | - | 324 | WP_223666488.1 | hypothetical protein | - |
| MWN28_RS26670 (MWN28_26680) | 15273..15413 | - | 141 | Protein_25 | hypothetical protein | - |
| MWN28_RS26675 (MWN28_26685) | 15433..16140 | - | 708 | WP_000108794.1 | hypothetical protein | - |
| MWN28_RS26680 (MWN28_26690) | 16142..16894 | - | 753 | WP_000797966.1 | conjugal transfer protein TraL | - |
| MWN28_RS26685 (MWN28_26695) | 16912..17313 | - | 402 | WP_021575837.1 | hypothetical protein | - |
| MWN28_RS26690 (MWN28_26700) | 17682..18023 | + | 342 | WP_000730722.1 | plasmid mobilization relaxosome protein MobC | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | blaCTX-M-64 / mcr-1.1 | - | 1..64620 | 64620 | |
| - | flank | IS/Tn | blaCTX-M-64 | - | 8062..10452 | 2390 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 83 a.a. Molecular weight: 9378.03 Da Isoelectric Point: 10.4800
>T242042 WP_000513532.1 NZ_CP095452:12722-12970 [Escherichia coli]
MGKADKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
VL
MGKADKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
VL
Download Length: 249 bp
>T242042 NZ_OV040719:369528-369749 [Haemophilus influenzae]
GTGCAAAATCTCCTTGAAAATCAAGCTCTTTTTGCTATTTTAGACGGTGAAATTTGGGAACTTCGTCCAATTAGGGATCG
CATCTTGTTTGCAAGATTAATGGACGGTCGCTTTGTCTTACTACATCAATTTATGAAGAAAACACAAAAAACGCCAAAAC
GAGAAATCCAAACTACCCAACAACGCTTAAGTGAATTAAAAGAGAGATTGAAAAATGAATAA
GTGCAAAATCTCCTTGAAAATCAAGCTCTTTTTGCTATTTTAGACGGTGAAATTTGGGAACTTCGTCCAATTAGGGATCG
CATCTTGTTTGCAAGATTAATGGACGGTCGCTTTGTCTTACTACATCAATTTATGAAGAAAACACAAAAAACGCCAAAAC
GAGAAATCCAAACTACCCAACAACGCTTAAGTGAATTAAAAGAGAGATTGAAAAATGAATAA
Antitoxin
Download Length: 116 a.a. Molecular weight: 13206.97 Da Isoelectric Point: 4.8313
>AT242042 WP_000681609.1 NZ_CP095452:12967-13314 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNILYGKLAFIRDLVTYEAETLADLEREFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSHHRAGA
MKHLKYKGYLGTVEPDFENNILYGKLAFIRDLVTYEAETLADLEREFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSHHRAGA
Download Length: 348 bp
>AT242042 NZ_OV040719:369742-370038 [Haemophilus influenzae]
ATGAATAAAATCAGCCCATTAGGTTCAAATTGGAATGAGTTTGAACAGCAAATTTTCAATGAAGAAGAAATCCGAGAAAG
TAATTTGCGTGTAGCCTTAATTAAAGAATTGATTACTTCTCGCCAACAACTTGGGATTTCCCAAAAACAACTTGAAACCT
TAAGCGGTGTGAAGCAACCTATGATTGCACGCATTGAAAAAGGACAAACGAATCCTCAGCTTGAAACGTTATTAAAATTG
CTCGCTCCTTTAGGGAAAACCCTGTCGATTGTGCCTCTAAGGGTAAAAAATGCCTAA
ATGAATAAAATCAGCCCATTAGGTTCAAATTGGAATGAGTTTGAACAGCAAATTTTCAATGAAGAAGAAATCCGAGAAAG
TAATTTGCGTGTAGCCTTAATTAAAGAATTGATTACTTCTCGCCAACAACTTGGGATTTCCCAAAAACAACTTGAAACCT
TAAGCGGTGTGAAGCAACCTATGATTGCACGCATTGAAAAAGGACAAACGAATCCTCAGCTTGAAACGTTATTAAAATTG
CTCGCTCCTTTAGGGAAAACCCTGTCGATTGTGCCTCTAAGGGTAAAAAATGCCTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A1I9ZJC5 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A1I9ZJ49 |