Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | TsxAB/MqsA(antitoxin) |
| Location | 7053..7546 | Replicon | plasmid pLS45-4 |
| Accession | NZ_CP095452 | ||
| Organism | Escherichia coli strain LS45 | ||
Toxin (Protein)
| Gene name | TsxA | Uniprot ID | U9Y9W8 |
| Locus tag | MWN28_RS26595 | Protein ID | WP_001230709.1 |
| Coordinates | 7053..7307 (+) | Length | 85 a.a. |
Antitoxin (Protein)
| Gene name | TsxB | Uniprot ID | U9Y4R8 |
| Locus tag | MWN28_RS26600 | Protein ID | WP_000151456.1 |
| Coordinates | 7304..7546 (+) | Length | 81 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| MWN28_RS26555 (MWN28_26565) | 2570..2722 | + | 153 | Protein_2 | DUF5431 family protein | - |
| MWN28_RS26560 (MWN28_26570) | 2667..2789 | + | 123 | WP_223685802.1 | Hok/Gef family protein | - |
| MWN28_RS26565 (MWN28_26575) | 2939..3535 | + | 597 | WP_000125165.1 | ProQ/FINO family protein | - |
| MWN28_RS26570 (MWN28_26580) | 3553..3837 | - | 285 | WP_001162371.1 | hypothetical protein | - |
| MWN28_RS26575 (MWN28_26585) | 3911..4576 | - | 666 | WP_000532149.1 | division plane positioning ATPase MipZ | - |
| MWN28_RS26580 (MWN28_26590) | 5165..5494 | + | 330 | WP_001542022.1 | hypothetical protein | - |
| MWN28_RS26585 (MWN28_26595) | 5895..6410 | + | 516 | WP_001025393.1 | J domain-containing protein | - |
| MWN28_RS26590 (MWN28_26600) | 6514..7056 | + | 543 | WP_001083904.1 | hypothetical protein | - |
| MWN28_RS26595 (MWN28_26605) | 7053..7307 | + | 255 | WP_001230709.1 | hypothetical protein | Toxin |
| MWN28_RS26600 (MWN28_26610) | 7304..7546 | + | 243 | WP_000151456.1 | hypothetical protein | Antitoxin |
| MWN28_RS26605 (MWN28_26615) | 7671..8015 | + | 345 | Protein_12 | WbuC family cupin fold metalloprotein | - |
| MWN28_RS26610 (MWN28_26620) | 8062..8937 | - | 876 | WP_032492042.1 | extended-spectrum class A beta-lactamase CTX-M-64 | - |
| MWN28_RS26615 (MWN28_26625) | 9190..10452 | - | 1263 | WP_000608644.1 | IS1380-like element ISEcp1 family transposase | - |
| MWN28_RS26620 (MWN28_26630) | 10852..11070 | + | 219 | WP_154101533.1 | hypothetical protein | - |
| MWN28_RS26625 (MWN28_26635) | 11202..11489 | + | 288 | WP_000673430.1 | hypothetical protein | - |
| MWN28_RS26630 (MWN28_26640) | 11541..11915 | + | 375 | WP_015387341.1 | hypothetical protein | - |
| MWN28_RS26635 (MWN28_26645) | 11930..12223 | + | 294 | WP_000954637.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | blaCTX-M-64 / mcr-1.1 | - | 1..64620 | 64620 | |
| - | flank | IS/Tn | blaCTX-M-64 | - | 8062..10452 | 2390 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 85 a.a. Molecular weight: 9942.47 Da Isoelectric Point: 10.1206
>T242041 WP_001230709.1 NZ_CP095452:7053-7307 [Escherichia coli]
MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTLSELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYK
ESDA
MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTLSELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYK
ESDA
Download Length: 255 bp
>T242041 NZ_OV040719:75122-75412 [Haemophilus influenzae]
ATGACTTATAAACTCATTTTTGATAAACGCGCATTAAAAGAATGGAATAAATTGGGGGAAACCTTACGCCAACAATTTAA
AAATAAATTAGTCGAAAGAATGATAAATCCACGTATTCAAGCCGACAAATTAAAGGGCGAAAATGATTTATATAAAATCA
AATTAAGAAGCGCTGGTTATCGATTAGTTTATCAAGTAAGAGATCAGGAAATCACCATTATTGTTGTATCTGTTGGAAAA
AGGGAACGATTAGAAGTCTATAAAACCGCAGAACAAAGAACAGCCCACTAA
ATGACTTATAAACTCATTTTTGATAAACGCGCATTAAAAGAATGGAATAAATTGGGGGAAACCTTACGCCAACAATTTAA
AAATAAATTAGTCGAAAGAATGATAAATCCACGTATTCAAGCCGACAAATTAAAGGGCGAAAATGATTTATATAAAATCA
AATTAAGAAGCGCTGGTTATCGATTAGTTTATCAAGTAAGAGATCAGGAAATCACCATTATTGTTGTATCTGTTGGAAAA
AGGGAACGATTAGAAGTCTATAAAACCGCAGAACAAAGAACAGCCCACTAA
Antitoxin
Download Length: 81 a.a. Molecular weight: 9136.45 Da Isoelectric Point: 7.2775
>AT242041 WP_000151456.1 NZ_CP095452:7304-7546 [Escherichia coli]
MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHNQLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP
MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHNQLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP
Download Length: 243 bp
>AT242041 NZ_OV040719:74878-75132 [Haemophilus influenzae]
ATGGCATTACATCAAATACTGGCAGAACAAGCGGCAAGCATTACAGACTTAAAACGAAACCCAATGGGCGTAATTCAAGA
AAGCGAAAGCGGAATAGTCGCAATATTGAACCGAAACCAACCCGCATTTTATTGCATCACGCCTGAATTATTTTCTCATA
TGCAAGAGCTAATCGAAGATTTAGAGCTCGGCAGAATAGCAAATGAACGGCTAGCCACCCTAGAACCTATAGAGGTTGAT
CTAAATGACTTATAA
ATGGCATTACATCAAATACTGGCAGAACAAGCGGCAAGCATTACAGACTTAAAACGAAACCCAATGGGCGTAATTCAAGA
AAGCGAAAGCGGAATAGTCGCAATATTGAACCGAAACCAACCCGCATTTTATTGCATCACGCCTGAATTATTTTCTCATA
TGCAAGAGCTAATCGAAGATTTAGAGCTCGGCAGAATAGCAAATGAACGGCTAGCCACCCTAGAACCTATAGAGGTTGAT
CTAAATGACTTATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3G9H8F0 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3G9GNF5 |