Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 53767..54410 Replicon plasmid pLS45-3
Accession NZ_CP095451
Organism Escherichia coli strain LS45

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag MWN28_RS26325 Protein ID WP_001044768.1
Coordinates 53767..54183 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag MWN28_RS26330 Protein ID WP_001261287.1
Coordinates 54180..54410 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MWN28_RS26310 (MWN28_26320) 50267..50857 - 591 WP_000194575.1 hypothetical protein -
MWN28_RS26315 (MWN28_26325) 50857..51114 - 258 WP_000343085.1 hypothetical protein -
MWN28_RS26320 (MWN28_26330) 51467..53605 + 2139 WP_000350638.1 AAA family ATPase -
MWN28_RS26325 (MWN28_26335) 53767..54183 - 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
MWN28_RS26330 (MWN28_26340) 54180..54410 - 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
MWN28_RS26335 (MWN28_26345) 54706..54996 + 291 WP_000111771.1 hypothetical protein -
MWN28_RS26340 (MWN28_26350) 54986..55885 + 900 WP_000963206.1 nucleotide-binding protein -
MWN28_RS26345 (MWN28_26355) 55935..56990 - 1056 WP_247180390.1 hypothetical protein -
MWN28_RS26350 (MWN28_26360) 56945..58162 - 1218 WP_247180391.1 P-loop NTPase fold protein -
MWN28_RS26355 (MWN28_26365) 58164..59252 - 1089 WP_000952217.1 transcriptional repressor PifC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaTEM-1B / rmtB / sul1 / qacE / aadA2 / dfrA12 / mph(A) / oqxA / oqxB / aph(3')-IIa / fosA3 / floR / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 adeG / adeG 1..92017 92017


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T242040 WP_001044768.1 NZ_CP095451:c54183-53767 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T242040 NZ_OV040584:c1569051-1568803 [Haemophilus influenzae]
ATGGGACGAACTGAAAAATTATTAGATAAGCTCGCACAATCAAAATCTACATTTAATTGGAATGAATTAGTTTCTTTGTT
AGCTCAACAAGGTTATGAAAAGCGAGAAATGGCAGGCTCTCGAGTGAGATTTTATAATAGAACACTCGAACATACGATTT
TGTTACACAAGCCTCACCCTGAAAATTATATTAAAGGTGGTGCTTTAAAGTCAGTGAAAGAATCATTAAAACAGGTAGGT
ATTCTATGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT242040 WP_001261287.1 NZ_CP095451:c54410-54180 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT242040 NZ_OV040584:c1568806-1568462 [Haemophilus influenzae]
ATGAAGTTATTAAATTATAAAGGTTATGTTGGCACGATTGAGGCGGATTTAGAAAACAATATATTGTTTGGCAAACTTGC
TTACATTCGTGATTTAGTGACTTACGAAGCAGAGTCATTATCTGAGCTAGAAAAAGAATTTCGTCAATCTGTTGATTTAT
ATTTACAAGATTGTTTGGAATTAGGTAAAGAACCGAATAAGCCTTTTAAAGGTGTATTTAATGTACGAATTGGCGAGGAA
TTGCATAGAGAAGCTACGATCATAGCAGGCGATCGTTCTCTTAATGCTTTTGTTACGGAAGCGATTCAAGAAAAAATTTT
TCGTGAAAAACCAAGTTTAAGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References