Detailed information of TA system TA024204

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 4327086..4327840
• Replicon: chromosome
• Accession: NC_013008
• Organism: Escherichia coli O157:H7 str. TW14359

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: ECSP_RS22700
• Protein ID: WP_001301452.1
• Coordinates: 4327355..4327840 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: Q8X700
• Locus tag: ECSP_RS22695
• Protein ID: WP_000801912.1
• Coordinates: 4327086..4327364 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECSP_RS22690	4324314..4327019	+	2706	WP_000906970.1	HTH-type transcriptional regulator MalT	-
ECSP_RS22695	4327086..4327364	+	279	WP_000801912.1	DUF1778 domain-containing protein	Antitoxin
ECSP_RS22700	4327355..4327840	+	486	WP_001301452.1	GNAT family N-acetyltransferase	Toxin
ECSP_RS22705	4327890..4328918	-	1029	WP_000827117.1	RNA 3'-terminal phosphate cyclase	-
ECSP_RS22710	4328922..4330148	-	1227	WP_001105473.1	RNA-splicing ligase RtcB	-
ECSP_RS22715	4330336..4331934	+	1599	WP_001232889.1	DNA-binding transcriptional regulator RtcR	-
ECSP_RS22720	4331916..4332674	-	759	WP_001302007.1	DeoR/GlpR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-92)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17650.57 Da
• Isoelectric Point: 9.4066

>T24204 WP_001301452.1 NC_013008:4327355-4327840 [Escherichia coli O157:H7 str. TW14359]
MGITAPTPLTSEHNLADFCCSDHGMNEWLKKKALKNHSSGLSRVYVICIANTRQVIGYYCLSTGSIQRNLAPGAMRRNAP
ESLPVVVLGRLAIDQAWAGKGLGVALLKDAVYRTMSIAQQVGVRALIVHALDDSVRNFYLKYAFVPSPFQSLTLLYPITL
E

Nucleotide

• Length: 486 bp

>T24204 NC_013008:4327355-4327840 [Escherichia coli O157:H7 str. TW14359]
ATGGGAATAACGGCTCCTACTCCCCTAACGTCTGAACATAATCTGGCTGACTTTTGCTGTTCCGATCACGGGATGAATGA
ATGGCTAAAAAAGAAAGCGTTAAAAAATCACAGCTCCGGTCTGTCTCGTGTTTACGTTATCTGTATTGCCAATACCCGCC
AGGTAATCGGCTATTACTGCCTTTCTACAGGCAGTATTCAACGTAATTTGGCCCCTGGAGCCATGCGACGTAATGCTCCC
GAATCGTTGCCTGTTGTCGTATTAGGCAGACTGGCAATTGATCAAGCCTGGGCAGGTAAAGGGTTGGGCGTCGCATTGTT
GAAAGATGCAGTCTATCGCACAATGTCCATAGCTCAACAGGTAGGGGTTCGTGCGCTCATTGTTCATGCCTTAGACGATT
CAGTACGCAATTTCTATCTGAAGTATGCCTTTGTACCTTCACCATTCCAGTCACTTACCTTACTGTATCCCATTACTCTG
GAGTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10420.94 Da
• Isoelectric Point: 4.6336

>AT24204 WP_000801912.1 NC_013008:4327086-4327364 [Escherichia coli O157:H7 str. TW14359]
MKPESKEAPINIRAKASQRDLIDMAANLVAKSRTDFMLDAACREAQDILLDQRLFILDDEQYDAFLAALDAPITAERQAK
INALMNRKSPWE

Nucleotide

• Length: 279 bp

>AT24204 NC_013008:4327086-4327364 [Escherichia coli O157:H7 str. TW14359]
ATGAAACCGGAAAGCAAAGAAGCGCCAATCAATATCAGAGCGAAAGCCTCACAGCGTGACCTGATTGATATGGCAGCGAA
TCTGGTCGCAAAATCGCGCACTGATTTTATGCTGGATGCCGCATGCCGTGAAGCGCAAGACATCTTATTAGATCAACGAC
TATTCATCCTTGATGACGAGCAATACGATGCGTTTCTCGCTGCCCTGGACGCCCCCATCACTGCTGAGCGTCAGGCCAAA
ATTAACGCGTTGATGAATCGTAAATCGCCATGGGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LHB9