Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4616940..4617437 Replicon chromosome
Accession NZ_CP095448
Organism Escherichia coli strain LS45

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag MWN28_RS22485 Protein ID WP_001173343.1
Coordinates 4616940..4617113 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag MWN28_RS22490 Protein ID WP_000398619.1
Coordinates 4617141..4617437 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MWN28_RS22475 (4613610) 4613610..4615067 + 1458 WP_000856843.1 dipeptide/tripeptide permease DtpC -
MWN28_RS22480 (4615304) 4615304..4616821 + 1518 WP_000003799.1 lysine--tRNA ligase -
MWN28_RS22485 (4616940) 4616940..4617113 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
MWN28_RS22490 (4617141) 4617141..4617437 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
MWN28_RS22495 (4617666) 4617666..4617938 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
MWN28_RS22500 (4617950) 4617950..4618180 - 231 WP_000371713.1 4Fe-4S mono-cluster protein YjdI -
MWN28_RS22505 (4618361) 4618361..4619992 + 1632 WP_001216471.1 sensor histidine kinase -
MWN28_RS22510 (4619989) 4619989..4620708 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4547940..4617437 69497


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T242036 WP_001173343.1 NZ_CP095448:c4617113-4616940 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T242036 NZ_OV040584:c323299-322991 [Haemophilus influenzae]
ATGTCAGAAGAGAAGCCATTAAAAGTGAGCTATTCAAAACAATTTGTAAGAGATTTAACTGATTTAGCTAAACGCTCCCC
CAATGTGTTAATTGGCTCAAAATACATTACTGCAATATACTGTTTATTAAATCGTTTACCTTTACCCGAAAACTACCAAG
ATCACGCATTAGTAGGAGAGTGGAAAGGTTATCGAGATTGCTATATTCAAGGCGATTTGGTACTGATTTACCAATACGTT
ATACACGATGAATTTGATGAATTGAAATTTTCTCGTTTAAATACACACTCACAAACCGCTTTAAAATAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT242036 WP_000398619.1 NZ_CP095448:c4617437-4617141 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT242036 NZ_OV040584:c323595-323299 [Haemophilus influenzae]
ATGGCACTTACGAATTCCTCTATCAGCTTTAGAACAGTTGAGAAAACAAAATTAGAAGCTTATCAAGTTATTGAACAATA
TGGACTTACCCCATCACAAGTATTCAATATGTTTTTGGCTCAAATTGCCAAGACTCGCTCTATCCCTGTTGATTTGAATT
ATCTTCGCCCAAATAAAGAAACCCTAGCAGCTATTGATGAATTAGATAGTGGTAACGCAGAGAGCTTCTTTATTGAAGCT
AGTGAAAATTATTCTGCCGAAGAATTTACAAAACGCATTCTTAATGGTGGTCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References