Detailed information of TA system TA242034

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 4446904..4447499
• Replicon: chromosome
• Accession: NZ_CP095448
• Organism: Escherichia coli strain LS45

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: V0SXT4
• Locus tag: MWN28_RS21640
• Protein ID: WP_000239581.1
• Coordinates: 4446904..4447254 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: L4JJX7
• Locus tag: MWN28_RS21645
• Protein ID: WP_001223213.1
• Coordinates: 4447248..4447499 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MWN28_RS21620 (4442358)	4442358..4443380	-	1023	WP_012311695.1	ABC transporter permease	-
MWN28_RS21625 (4443394)	4443394..4444896	-	1503	WP_247180321.1	sugar ABC transporter ATP-binding protein	-
MWN28_RS21630 (4445029)	4445029..4445985	-	957	WP_000265933.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
MWN28_RS21635 (4446295)	4446295..4446825	+	531	WP_000055075.1	inorganic diphosphatase	-
MWN28_RS21640 (4446904)	4446904..4447254	-	351	WP_000239581.1	endoribonuclease toxin ChpB	Toxin
MWN28_RS21645 (4447248)	4447248..4447499	-	252	WP_001223213.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
MWN28_RS21650 (4447711)	4447711..4448052	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
MWN28_RS21655 (4448055)	4448055..4451834	-	3780	WP_000060872.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12453.41 Da
• Isoelectric Point: 6.2206

>T242034 WP_000239581.1 NZ_CP095448:c4447254-4446904 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T242034 NZ_OV024757:1170491-1170904 [Deinococcus radiodurans]
ATGACAGAGAGCACCGTGCTGGACGCCAATATTCTCAGTGCCCTGCTCCGTTCTGAGGCGAACCCTCTGTCCATTGCGCG
TCAGCTTTCGGCGCTGGCCCGTGAGGGTTCACTAGTGGTTTATGCCGAGTTGCTCGCGGGCCCTGAGGTTACGCCCGATT
TCCTCAAGGGATTTCTGCAAGAGGTGGACGTGCAGATTCTTCCGCCGTCTGGCCTTGCGGTCTGGGAAAGCGCGGGCCTC
GCTTATGGGAGCTACGCTCAGCGGCGGCAACGCAGCGGTGGTGGGTCGCCTCGCCAGATTCTGAGTGACTTTGTGATTGG
TGCCCATGCCCTGTATCACGGCGCCGCTCTCTTCACCGCTGATCCTCAGCATTACCGCCTCAGCTTCCCAGCCCTCACAG
TCCTGACCCCATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9331.64 Da
• Isoelectric Point: 4.6509

>AT242034 WP_001223213.1 NZ_CP095448:c4447499-4447248 [Escherichia coli]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT242034 NZ_OV024757:1170270-1170494 [Deinococcus radiodurans]
ATGACGACGTTGGAGGCCCGGATTGAGAACGGCAAGGTGGTGCTCACCCCTGAGCAGGCCGCCGAACTGGGCCTGCGCGA
AGGCGAAGTGCTGCCGGTGACGATTGCGCCGCACGAAGCGGAAACGGAAGGCAACCCCTTCACCCGCTGGATTGGGACGC
TCCCCCCACTGCCTGACGGTGAAGAGTCTGCGCGGTTCTACCGCCGCCTGCGAGACGGCGAATGA

Structures

Toxin

• AlphaFold DB: V0SXT4

Antitoxin

• AlphaFold DB: L4JJX7