Detailed information of TA system TA242027

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3686302..3687139
• Replicon: chromosome
• Accession: NZ_CP095448
• Organism: Escherichia coli strain LS45

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: V0SED1
• Locus tag: MWN28_RS17810
• Protein ID: WP_000227787.1
• Coordinates: 3686597..3687139 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: B1LJI1
• Locus tag: MWN28_RS17805
• Protein ID: WP_001353405.1
• Coordinates: 3686302..3686613 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MWN28_RS17780 (3681322)	3681322..3682269	+	948	WP_001239436.1	cytochrome o ubiquinol oxidase subunit II	-
MWN28_RS17785 (3682291)	3682291..3684282	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
MWN28_RS17790 (3684272)	3684272..3684886	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
MWN28_RS17795 (3684886)	3684886..3685215	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
MWN28_RS17800 (3685227)	3685227..3686117	+	891	WP_000971336.1	heme o synthase	-
MWN28_RS17805 (3686302)	3686302..3686613	+	312	WP_001353405.1	DUF1778 domain-containing protein	Antitoxin
MWN28_RS17810 (3686597)	3686597..3687139	+	543	WP_000227787.1	GNAT family N-acetyltransferase	Toxin
MWN28_RS17815 (3687195)	3687195..3688130	-	936	WP_012311701.1	tetratricopeptide repeat protein	-
MWN28_RS17820 (3688537)	3688537..3689901	+	1365	WP_001000978.1	MFS transporter	-
MWN28_RS17825 (3690029)	3690029..3690520	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
MWN28_RS17830 (3690688)	3690688..3691599	+	912	WP_000705871.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (19-96)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19946.24 Da
• Isoelectric Point: 8.8951

>T242027 WP_000227787.1 NZ_CP095448:3686597-3687139 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHRALPIKGVYLDADPAAINFYTRLGFVQLSA
RPNVFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T242027 NZ_OU943338:4651249-4651560 [Salmonella enterica subsp. enterica serovar Typhi]
GTGCATGTTATCAGCCGAAAACTGTTCAATGAAGCGATGCTCATGTATCCAAACCATGAGCTTGCTCTCACTGAGTTGCT
GAATGTTCTGGAGAAAAAAACATTCACTCAGCCTGAAGAGATGAAGCGGTATATTCCTTCGCTGGATAATTTTAAGTACA
GAGATAAATGGTGGGTGATTGATGTTTCTGGTAACAGCCTGAGACTCATTTCCTACATAGATTTCAGGTTACATAAGATA
TTTGTGAAACATATCGTTTCCCATGCCGAATATGACAAACTGACCGCATATTATCGGGGTAATGAAGAATGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11370.09 Da
• Isoelectric Point: 10.4825

>AT242027 WP_001353405.1 NZ_CP095448:3686302-3686613 [Escherichia coli]
MPGKTVALPDIDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT242027 NZ_OU943338:4651557-4652003 [Salmonella enterica subsp. enterica serovar Typhi]
ATGAGAACTCATCGTCAGATGGATGCAACCAGCGCAAAAAAGATCGTTGATACCTTCAGCGATGCGGTAAAAAGCGTCCC
ACTGATGGGGGAAGACCGAAATGACAATGAGTATCGCAGGGCACTAGCGCTAGTGGAGTTTCTGGTCGACCACGACGATC
TTGAAAACCCACTATTTGAATTGCTCTGTGCCCGAATCAGTGAATACGAAAAACATGCGCCGGAATTCAAAGCACTCAAC
CAACACCTGGAGAAAACGCCCCCTGGCGTTTCAGTATTGCGAACGCTCATGGATCAATACGGTCTCAAAGCAGCAGATCT
TGCCAACGAACTTGGTTCTAAATCGAACGTCAGCAACATCTTAAATGGCCGCAGAGCACTAACGGTTAATCATATTAAAG
CGCTTACACAACGCTTTAAACTACCAGCGGATGCCTTCATCGAGTAG

Structures

Toxin

• AlphaFold DB: A0A829GE43

Antitoxin

• AlphaFold DB: A0A829G7G3