Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3652213..3652831 Replicon chromosome
Accession NZ_CP095448
Organism Escherichia coli strain LS45

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag MWN28_RS17640 Protein ID WP_001291435.1
Coordinates 3652613..3652831 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag MWN28_RS17635 Protein ID WP_000344800.1
Coordinates 3652213..3652587 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MWN28_RS17625 (3647302) 3647302..3648495 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
MWN28_RS17630 (3648518) 3648518..3651667 + 3150 WP_072754545.1 efflux RND transporter permease AcrB -
MWN28_RS17635 (3652213) 3652213..3652587 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
MWN28_RS17640 (3652613) 3652613..3652831 + 219 WP_001291435.1 HHA domain-containing protein Toxin
MWN28_RS17645 (3653003) 3653003..3653554 + 552 WP_000102561.1 maltose O-acetyltransferase -
MWN28_RS17650 (3653670) 3653670..3654140 + 471 WP_000136192.1 YlaC family protein -
MWN28_RS17655 (3654304) 3654304..3655854 + 1551 WP_072754547.1 cyclic-guanylate-specific phosphodiesterase PdeB -
MWN28_RS17660 (3655895) 3655895..3656248 - 354 WP_000878140.1 DUF1428 domain-containing protein -
MWN28_RS17670 (3656627) 3656627..3656938 + 312 WP_000409911.1 MGMT family protein -
MWN28_RS17675 (3656969) 3656969..3657541 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T242026 WP_001291435.1 NZ_CP095448:3652613-3652831 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T242026 NZ_OU943338:c4649787-4649413 [Salmonella enterica subsp. enterica serovar Typhi]
ATGGTCAAAGGGTCTGCGCTTTTTGATACCAATATCCTCATCGACCTGTTTAGCGGTCGAATTGAAGCGAAACATGCGCT
GGAAGCGTATCCGCCACAAAATGCGATTAGCCTTATCACATGGATGGAAGTGATGGTGGGTGCTAAGAAATATCATCAGG
AAAACCGCACGCGCATTGCATTAAGTGCGTTCAATATTATTGGCGTAACACAGGAAATCGCGGAACGAAGCGTCATTGTC
AGGCAGGAGTATGGCATGAAACTCCCGGATGCGATCATTCTGGCCACTGCACAAGTCCATCGTTGTGAGCTTGTGACGCG
CAATACAAAAGACTTTGCCGATATTCCGGGAGTGATCACGCCGTATCACTTGTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT242026 WP_000344800.1 NZ_CP095448:3652213-3652587 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT242026 NZ_OU943338:c4650029-4649787 [Salmonella enterica subsp. enterica serovar Typhi]
ATGATGGCTGGGATGGATATGGGCAGAATTTTACTCGATTTATCAGACGATGTGATTAAACGGCTTGACGATCTCAAGGT
GCAACGCAACCTACCTCGTGCAGAACTCTTGCGGGAGGCCGTTGAGCAGTATCTGGAAAGACAGGATCGGGCTGAGACCA
CAATTTCGAAAGCATTGGGGCTCTGGCAAGGCTGTGAGGAAGATGGCGTTGAATACCAACGTAAACTTCGTGAGGAATGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References