Detailed information of TA system TA242025

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3293332..3294037
• Replicon: chromosome
• Accession: NZ_CP095448
• Organism: Escherichia coli strain LS45

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: MWN28_RS15910
• Protein ID: WP_000539521.1
• Coordinates: 3293332..3293718 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: MWN28_RS15915
• Protein ID: WP_001280945.1
• Coordinates: 3293708..3294037 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MWN28_RS15890 (3289336)	3289336..3289962	+	627	WP_001295292.1	glutathione S-transferase GstB	-
MWN28_RS15895 (3289959)	3289959..3291074	-	1116	WP_000555050.1	aldose sugar dehydrogenase YliI	-
MWN28_RS15900 (3291185)	3291185..3291568	-	384	WP_000497137.1	biofilm formation regulator BssR	-
MWN28_RS15905 (3291781)	3291781..3293106	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
MWN28_RS15910 (3293332)	3293332..3293718	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MWN28_RS15915 (3293708)	3293708..3294037	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
MWN28_RS15920 (3294107)	3294107..3295435	-	1329	WP_000086869.1	GGDEF domain-containing protein	-
MWN28_RS15925 (3295443)	3295443..3297790	-	2348	Protein_3133	EAL domain-containing protein	-
MWN28_RS15930 (3297967)	3297967..3298878	-	912	WP_001236058.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T242025 WP_000539521.1 NZ_CP095448:3293332-3293718 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T242025 NZ_OU943338:c4264822-4264331 [Salmonella enterica subsp. enterica serovar Typhi]
ATGATCTCCACCCCTGAGCCGCTTCATGCCGGACATATTCTTACTCCGTTTTGCTGCGGTGTGGATTCTATAGATAACTG
GCTGAAACAGCGGGCGATGAAAAATCAGACCACTGGCGCGTCCCGTACCTTTGTGTGTTGTGGCAGCGATTCGAACGTAC
TGGCCTATTACTCGCTGGCGTCCAGCGCGGTCACGACGAATACCTCCCCCGGTCGCTTTCGTCGCAATATGCCTGACCCG
ATTCCGGTTGTGGTATTGGGGCGTCTGGCGGTGGATAAATCGCTTCATGGGCAGGGCGTCGCTCGGGCGCTGGTACGCGA
CGCAGGGCTGCGGGTTATTCAGGTGGCGGAGACTATCGGCATTCGTGGGATGCTGGTTCACGCCCTGTCGGATGAAGCGC
GGGAATTTTATCAGTGGGTGGGGTTTGTACCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGGGGGATTTGGTG
GAGAGTGTTTAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT242025 WP_001280945.1 NZ_CP095448:3293708-3294037 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT242025 NZ_OU943338:c4265112-4264819 [Salmonella enterica subsp. enterica serovar Typhi]
ATGCCAGCCGCAAACAGTATGGCGATGAAACGTGAAACTCTGAACCTGCGTATCAAACCCGCCGAGCGCGATCTTATTGA
TCGAGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGTTGGAAGCTGCCCGTGCTGCCGCAGAAGAAGTGCTTA
TCGAACAACGCATCATTATGGCCGATCCAGAGGCTTATCAGGAATTTCTCGTTCGTTTGGATCAAACACCTTCACCTAAT
GCCGCACTGCGTAAAACCATGCAGACCCCTGCGCCGTGGGAGCAGGAAAAATGA

Structures

Toxin

• AlphaFold DB: A0A829GCT6