Detailed information of TA system TA242016

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2426531..2427169
• Replicon: chromosome
• Accession: NZ_CP095448
• Organism: Escherichia coli strain LS45

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: MWN28_RS11590
• Protein ID: WP_000813794.1
• Coordinates: 2426531..2426707 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: MWN28_RS11595
• Protein ID: WP_001270286.1
• Coordinates: 2426753..2427169 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MWN28_RS11570 (2422444)	2422444..2423325	-	882	Protein_2272	BenE family transporter YdcO	-
MWN28_RS11575 (2423417)	2423417..2423953	+	537	WP_000429154.1	DNA-binding transcriptional regulator SutR	-
MWN28_RS11580 (2424026)	2424026..2425987	+	1962	WP_012311648.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
MWN28_RS11585 (2426079)	2426079..2426309	-	231	WP_000491567.1	DUF2554 family protein	-
MWN28_RS11590 (2426531)	2426531..2426707	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
MWN28_RS11595 (2426753)	2426753..2427169	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
MWN28_RS11600 (2427248)	2427248..2428654	+	1407	WP_000760611.1	PLP-dependent aminotransferase family protein	-
MWN28_RS11605 (2428898)	2428898..2430043	+	1146	WP_000034364.1	ABC transporter substrate-binding protein	-
MWN28_RS11610 (2430061)	2430061..2431074	+	1014	WP_000220391.1	ABC transporter ATP-binding protein	-
MWN28_RS11615 (2431075)	2431075..2432016	+	942	WP_001251303.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2421102..2422373	1271

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T242016 WP_000813794.1 NZ_CP095448:2426531-2426707 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T242016 NZ_OU943338:204472-204957 [Salmonella enterica subsp. enterica serovar Typhi]
GTGGGACGTGTAACAGCACCAGAACCTTTGTCCGCTTTTCATCAGGTAGCTGAGTTCGTCAGCGGTGAAGCTGTGCTCGA
TGACTGGTTGAAGCAAAAGGGCCTCAAAAACCAGGCTCTCGGAGCGGCCAGAACATTTGTGGTGTGCAAGAAAGACACGA
AGCAAGTAGCCGGTTTTTACTCTCTGGCCACCGGTAGCGTCAACCATACAGAAGCGACAGGCAACCTTCGGCGTAACATG
CCAGATCCCATCCCTGTCATTATACTTGCCCGTCTTGCTGTCGATCTCTCATTCCATGGAAAAGGGCTTGGGGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTTACCG
AAGACGCCAAAAATTTCTACATTCACCATGGTTTCAAATCATCACAAACTCAGCAGCGAACATTGTTCCTTAGGCTCCCT
CAATAG

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT242016 WP_001270286.1 NZ_CP095448:2426753-2427169 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT242016 NZ_OU943338:204197-204484 [Salmonella enterica subsp. enterica serovar Typhi]
ATGCTATACAAGGGGTGTCTCATGAAATCAGATGTTCAACTTAACCTTAGAGCTAAGGAGTCGCAGCGGGCGCTCATTGA
TGCAGCTGCGGAAATCCTTCACAAGTCACGTACAGATTTCATTCTGGAAACGGCCTGCCGGGCTGCCGAGAATGTGATCC
TTGACCGCCGTGTATTTAACTTTAACGATGAGCAATATGAGGAGTTCATCAATCTGCTTGATGCACCGGTCGCAGATGAT
CCCGTTATCGAAAAACTGCTGGCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Toxin

• PDB: 6HPB