Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1141664..1142247 Replicon chromosome
Accession NZ_CP095448
Organism Escherichia coli strain LS45

Toxin (Protein)


Gene name mazF Uniprot ID V0SV58
Locus tag MWN28_RS05435 Protein ID WP_000254750.1
Coordinates 1141912..1142247 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag MWN28_RS05430 Protein ID WP_000581937.1
Coordinates 1141664..1141912 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MWN28_RS05420 (1138003) 1138003..1139304 + 1302 WP_000046808.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
MWN28_RS05425 (1139352) 1139352..1141586 + 2235 WP_000226795.1 GTP diphosphokinase -
MWN28_RS05430 (1141664) 1141664..1141912 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
MWN28_RS05435 (1141912) 1141912..1142247 + 336 WP_000254750.1 endoribonuclease MazF Toxin
MWN28_RS05440 (1142319) 1142319..1143108 + 790 Protein_1069 nucleoside triphosphate pyrophosphohydrolase -
MWN28_RS05445 (1143336) 1143336..1144973 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
MWN28_RS05450 (1145060) 1145060..1146358 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12067.95 Da        Isoelectric Point: 8.2618

>T242010 WP_000254750.1 NZ_CP095448:1141912-1142247 [Escherichia coli]
MVSRYVPDTGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T242010 NZ_OU943337:c3922368-3922081 [Salmonella enterica subsp. enterica serovar Typhi]
ATGCCGATGGAGTTTGAATGGGATGCGAACAAGGCTAAAAGCAACCTGAGGAAGCACGGTGTCCGCTTTGAGGATGCGGT
ACTGGTGTTTGACGATCCCCGACACCTGTCACGTCAGGAGCGTTATGAGAATGGAGAATATCGTTGGCAGACCCTCGGCC
TTGTTCATGGCATTGTGGTCATTCTGGTTGCTCATAGCGTTCGGTTCGAAAGTGGTTTTGAAGTTATCCGTATCATCAGC
GCTCGTAAGGCAGACAGAAAAGAGAGGAATCGTTATGAGCATGGTTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT242010 WP_000581937.1 NZ_CP095448:1141664-1141912 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT242010 NZ_OU943337:c3922094-3921792 [Salmonella enterica subsp. enterica serovar Typhi]
ATGAGCATGGTTAAACATAAACGCGGCAACGCATCCGCGCTGAGTGCTCAGCATGAGGCTGAACTGAAGGCGCTGGCGAA
AAAATCAGATGATGAGATTGATTACAGCGACATTCCCGCTTCGGAGGATGGACAGTGGTCTGAAGCAGTGCGTGGTAAGT
TTTTTCGCCCGTTAAAAACTCAGGCCTCGGTGCGTATTGACGCCGACGTTATGGAATGGCTGAAACGGCCAGGAAAAGGC
TACCAGACGCGGCTGAATGCCATTCTGCGCGAAGCGATGCTGCGCGAGCAAAATAAGAAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SV58

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References