Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1039965..1040619 Replicon chromosome
Accession NZ_CP095448
Organism Escherichia coli strain LS45

Toxin (Protein)


Gene name cptA Uniprot ID F4T2L4
Locus tag MWN28_RS04990 Protein ID WP_000244765.1
Coordinates 1040212..1040619 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag MWN28_RS04985 Protein ID WP_000354046.1
Coordinates 1039965..1040231 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MWN28_RS04965 (1036053) 1036053..1037486 - 1434 WP_049039059.1 6-phospho-beta-glucosidase BglA -
MWN28_RS04970 (1037531) 1037531..1037842 + 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
MWN28_RS04975 (1038006) 1038006..1038665 + 660 WP_000250274.1 hemolysin III family protein -
MWN28_RS04980 (1038742) 1038742..1039722 - 981 WP_049038218.1 tRNA-modifying protein YgfZ -
MWN28_RS04985 (1039965) 1039965..1040231 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
MWN28_RS04990 (1040212) 1040212..1040619 + 408 WP_000244765.1 protein YgfX Toxin
MWN28_RS04995 (1040659) 1040659..1041180 - 522 WP_001055874.1 flavodoxin FldB -
MWN28_RS05000 (1041292) 1041292..1042188 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
MWN28_RS05005 (1042213) 1042213..1042923 + 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
MWN28_RS05010 (1042929) 1042929..1044662 + 1734 WP_000813190.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16043.95 Da        Isoelectric Point: 11.5202

>T242009 WP_000244765.1 NZ_CP095448:1040212-1040619 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLIPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T242009 NZ_OU943337:3342121-3342339 [Salmonella enterica subsp. enterica serovar Typhi]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT242009 WP_000354046.1 NZ_CP095448:1039965-1040231 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT242009 NZ_OU943337:3341719-3342093 [Salmonella enterica subsp. enterica serovar Typhi]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A454A7D7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References