Detailed information of TA system TA242008

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 875388..876223
• Replicon: chromosome
• Accession: NZ_CP095448
• Organism: Escherichia coli strain LS45

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1EZ92
• Locus tag: MWN28_RS04145
• Protein ID: WP_000854726.1
• Coordinates: 875388..875765 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: MWN28_RS04150
• Protein ID: WP_049043351.1
• Coordinates: 875855..876223 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MWN28_RS04115 (870502)	870502..871650	-	1149	WP_000905926.1	capsule polysaccharide export inner-membrane protein KpsE	-
MWN28_RS04120 (871722)	871722..872705	-	984	WP_001298261.1	KpsF/GutQ family sugar-phosphate isomerase	-
MWN28_RS04125 (873516)	873516..873686	-	171	Protein_811	IS110 family transposase	-
MWN28_RS04130 (874028)	874028..874597	-	570	WP_016231187.1	DUF4942 domain-containing protein	-
MWN28_RS04135 (874694)	874694..874891	-	198	WP_016231186.1	DUF957 domain-containing protein	-
MWN28_RS04140 (874903)	874903..875391	-	489	WP_072754450.1	DUF5983 family protein	-
MWN28_RS04145 (875388)	875388..875765	-	378	WP_000854726.1	TA system toxin CbtA family protein	Toxin
MWN28_RS04150 (875855)	875855..876223	-	369	WP_049043351.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
MWN28_RS04155 (876273)	876273..876917	-	645	Protein_817	antitoxin of toxin-antitoxin stability system	-
MWN28_RS04160 (876932)	876932..877153	-	222	WP_000692346.1	DUF987 domain-containing protein	-
MWN28_RS04165 (877216)	877216..877692	-	477	WP_001186774.1	RadC family protein	-
MWN28_RS04170 (877708)	877708..878193	-	486	WP_021547706.1	antirestriction protein	-
MWN28_RS04175 (878285)	878285..879103	-	819	WP_021547707.1	DUF932 domain-containing protein	-
MWN28_RS04180 (879193)	879193..879426	-	234	WP_001278282.1	DUF905 family protein	-
MWN28_RS04185 (879432)	879432..880109	-	678	WP_001097567.1	hypothetical protein	-
MWN28_RS04190 (880260)	880260..880940	-	681	WP_001282912.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14164.10 Da
• Isoelectric Point: 7.8045

>T242008 WP_000854726.1 NZ_CP095448:c875765-875388 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITQGKHPEAKQ

Nucleotide

• Length: 378 bp

>T242008 NZ_OU943337:1974593-1974695 [Salmonella enterica subsp. enterica serovar Typhi]
GCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13550.23 Da
• Isoelectric Point: 5.8786

>AT242008 WP_049043351.1 NZ_CP095448:c876223-875855 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGVTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT242008 NZ_OU943337:c1974697-1974554 [Salmonella enterica subsp. enterica serovar Typhi]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCTCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTG

Structures

Toxin

• AlphaFold DB: S1EZ92