Detailed information of TA system TA242004

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 865223..865873
• Replicon: chromosome
• Accession: NZ_CP095443
• Organism: Providencia stuartii strain CMC-4104

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: K8W3H1
• Locus tag: MWH14_RS04065
• Protein ID: WP_004927064.1
• Coordinates: 865223..865426 (-)
• Length: 68 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: MWH14_RS04070
• Protein ID: WP_154635730.1
• Coordinates: 865505..865873 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MWH14_RS04040 (MWH14_04040)	861103..861441	+	339	WP_154622397.1	P-II family nitrogen regulator	-
MWH14_RS04045 (MWH14_04045)	861452..862735	+	1284	WP_154622396.1	ammonium transporter AmtB	-
MWH14_RS04050 (MWH14_04050)	862963..863829	-	867	WP_154622395.1	acyl-CoA thioesterase II	-
MWH14_RS04055 (MWH14_04055)	864154..864612	+	459	WP_154622394.1	YbaY family lipoprotein	-
MWH14_RS04065 (MWH14_04065)	865223..865426	-	204	WP_004927064.1	HHA domain-containing protein	Toxin
MWH14_RS04070 (MWH14_04070)	865505..865873	-	369	WP_154635730.1	Hha toxicity modulator TomB	Antitoxin
MWH14_RS04075 (MWH14_04075)	866441..867778	-	1338	WP_154624041.1	murein transglycosylase D	-
MWH14_RS04080 (MWH14_04080)	867857..868612	-	756	WP_154624042.1	hydroxyacylglutathione hydrolase	-
MWH14_RS04085 (MWH14_04085)	868652..869377	+	726	WP_154624043.1	methyltransferase domain-containing protein	-
MWH14_RS04090 (MWH14_04090)	869444..869914	-	471	WP_154624044.1	ribonuclease HI	-
MWH14_RS04095 (MWH14_04095)	869969..870730	+	762	WP_154624045.1	DNA polymerase III subunit epsilon	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (8-64)

Antitoxin

• Biofilm formation regulator YbaJ (1-117)

Sequences

Toxin

Protein

• Length: 68 a.a.
• Molecular weight: 8075.37 Da
• Isoelectric Point: 6.9770

>T242004 WP_004927064.1 NZ_CP095443:c865426-865223 [Providencia stuartii]
MTKTDYLMRLRKCTTIDTLERVIEKNKYELSEDELELFYSAADHRLAELTMNKLYDKIPASVWKFVR

Nucleotide

• Length: 204 bp

>T242004 NZ_OU943337:204472-204957 [Salmonella enterica subsp. enterica serovar Typhi]
GTGGGACGTGTAACAGCACCAGAACCTTTGTCCGCTTTTCATCAGGTAGCTGAGTTCGTCAGCGGTGAAGCTGTGCTCGA
TGACTGGTTGAAGCAAAAGGGCCTCAAAAACCAGGCTCTCGGAGCGGCCAGAACATTTGTGGTGTGCAAGAAAGACACGA
AGCAAGTAGCCGGTTTTTACTCTCTGGCCACCGGTAGCGTCAACCATACAGAAGCGACAGGCAACCTTCGGCGTAACATG
CCAGATCCCATCCCTGTCATTATACTTGCCCGTCTTGCTGTCGATCTCTCATTCCATGGAAAAGGGCTTGGGGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTTACCG
AAGACGCCAAAAATTTCTACATTCACCATGGTTTCAAATCATCACAAACTCAGCAGCGAACATTGTTCCTTAGGCTCCCT
CAATAG

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 14106.92 Da
• Isoelectric Point: 4.3486

>AT242004 WP_154635730.1 NZ_CP095443:c865873-865505 [Providencia stuartii]
MDEYSPKNYDISELKYLCNSLNREAISSLQKTNTHWINDLSSPQSVRLNELIEHIAAFVWQYKIKHPKDNLVISLVEEYL
DETYDLFGSPVITLSEIIDWQSMNQNLVAVLDDDLKCPASKT

Nucleotide

• Length: 369 bp

>AT242004 NZ_OU943337:204197-204484 [Salmonella enterica subsp. enterica serovar Typhi]
ATGCTATACAAGGGGTGTCTCATGAAATCAGATGTTCAACTTAACCTTAGAGCTAAGGAGTCGCAGCGGGCGCTCATTGA
TGCAGCTGCGGAAATCCTTCACAAGTCACGTACAGATTTCATTCTGGAAACGGCCTGCCGGGCTGCCGAGAATGTGATCC
TTGACCGCCGTGTATTTAACTTTAACGATGAGCAATATGAGGAGTTCATCAATCTGCTTGATGCACCGGTCGCAGATGAT
CCCGTTATCGAAAAACTGCTGGCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Toxin

• AlphaFold DB: K8W3H1