Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 31998..32523 Replicon plasmid pIM007_ESBL
Accession NZ_CP095430
Organism Klebsiella pneumoniae strain IM007

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag MWR71_RS29110 Protein ID WP_013023785.1
Coordinates 32218..32523 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag MWR71_RS29105 Protein ID WP_001568025.1
Coordinates 31998..32216 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MWR71_RS29085 (MWR71_29035) 27166..27945 + 780 WP_013023780.1 hypothetical protein -
MWR71_RS29090 (MWR71_29040) 28221..29744 + 1524 WP_017899887.1 hypothetical protein -
MWR71_RS29095 (MWR71_29045) 29778..30905 + 1128 WP_013023782.1 DUF4238 domain-containing protein -
MWR71_RS29100 (MWR71_29050) 30902..31180 + 279 WP_271237394.1 korC -
MWR71_RS29105 (MWR71_29055) 31998..32216 + 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
MWR71_RS29110 (MWR71_29060) 32218..32523 + 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
MWR71_RS29115 (MWR71_29065) 32692..33087 + 396 WP_017899885.1 hypothetical protein -
MWR71_RS29120 (MWR71_29070) 33114..33428 + 315 WP_053389906.1 hypothetical protein -
MWR71_RS29125 (MWR71_29075) 33439..34455 + 1017 WP_017899884.1 hypothetical protein -
MWR71_RS29130 (MWR71_29080) 34653..35447 + 795 WP_004197635.1 site-specific integrase -
MWR71_RS29135 (MWR71_29085) 35895..36197 - 303 WP_004197636.1 hypothetical protein -
MWR71_RS29140 (MWR71_29090) 36194..36820 - 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid mph(A) / tet(A) / floR / qnrS1 / blaCTX-M-3 / blaTEM-1B / aac(6')-Ib-cr / ARR-3 / dfrA27 / aadA16 / sul1 - 1..124551 124551


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T241998 WP_013023785.1 NZ_CP095430:32218-32523 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T241998 NZ_OU943336:c3922503-3922216 [Salmonella enterica subsp. enterica serovar Typhi]
ATGCCGATGGAGTTTGAATGGGATGCGAACAAGGCTAAAAGCAACCTGAGGAAGCACGGTGTCCGCTTTGAGGATGCGGT
ACTGGTGTTTGACGATCCCCGACACCTGTCACGTCAGGAGCGTTATGAGAATGGAGAATATCGTTGGCAGACCCTCGGCC
TTGTTCATGGCATTGTGGTCATTCTGGTTGCTCATAGCGTTCGGTTCGAAAGTGGTTTTGAAGTTATCCGTATCATCAGC
GCTCGTAAGGCAGACAGAAAAGAGAGGAATCGTTATGAGCATGGTTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT241998 WP_001568025.1 NZ_CP095430:31998-32216 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT241998 NZ_OU943336:c3922229-3921927 [Salmonella enterica subsp. enterica serovar Typhi]
ATGAGCATGGTTAAACATAAACGCGGCAACGCATCCGCGCTGAGTGCTCAGCATGAGGCTGAACTGAAGGCGCTGGCGAA
AAAATCAGATGATGAGATTGATTACAGCGACATTCCCGCTTCGGAGGATGGACAGTGGTCTGAAGCAGTGCGTGGTAAGT
TTTTTCGCCCGTTAAAAACTCAGGCCTCGGTGCGTATTGACGCCGACGTTATGGAATGGCTGAAACGGCCAGGAAAAGGC
TACCAGACGCGGCTGAATGCCATTCTGCGCGAAGCGATGCTGCGCGAGCAAAATAAGAAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References