Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 193065..193735 Replicon plasmid pIM007_vir
Accession NZ_CP095429
Organism Klebsiella pneumoniae strain IM007

Toxin (Protein)


Gene name vagD Uniprot ID Q6U619
Locus tag MWR71_RS28715 Protein ID WP_004213072.1
Coordinates 193292..193735 (+) Length 148 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q6U620
Locus tag MWR71_RS28710 Protein ID WP_004213073.1
Coordinates 193065..193295 (+) Length 77 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MWR71_RS28685 (MWR71_28635) 189288..190187 - 900 WP_004225022.1 nucleotide-binding protein -
MWR71_RS28690 (MWR71_28640) 190177..190467 - 291 WP_004213078.1 hypothetical protein -
MWR71_RS28695 (MWR71_28645) 190819..191025 + 207 WP_004213077.1 hypothetical protein -
MWR71_RS28700 (MWR71_28650) 191015..191308 - 294 WP_004213076.1 hypothetical protein -
MWR71_RS28705 (MWR71_28655) 191324..192457 - 1134 WP_004213075.1 DUF3800 domain-containing protein -
MWR71_RS28710 (MWR71_28660) 193065..193295 + 231 WP_004213073.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
MWR71_RS28715 (MWR71_28665) 193292..193735 + 444 WP_004213072.1 type II toxin-antitoxin system VapC family toxin Toxin
MWR71_RS28720 (MWR71_28670) 193884..194135 + 252 WP_186987481.1 hypothetical protein -
MWR71_RS28725 (MWR71_28675) 194158..194462 - 305 Protein_207 transposase -
MWR71_RS28730 (MWR71_28680) 194600..195580 + 981 WP_000019473.1 IS5-like element ISKpn26 family transposase -
MWR71_RS28735 (MWR71_28685) 195744..196304 + 561 WP_004213927.1 TetR/AcrR family transcriptional regulator -
MWR71_RS28740 (MWR71_28690) 196342..196770 + 429 WP_004902152.1 DUF417 family protein -
MWR71_RS28745 (MWR71_28695) 196854..197129 + 276 WP_004213932.1 carboxymuconolactone decarboxylase family protein -
MWR71_RS28750 (MWR71_28700) 197192..197683 + 492 WP_011251327.1 DM13 domain-containing protein -
MWR71_RS28755 (MWR71_28705) 197732..198652 + 921 WP_004213934.1 DUF1471 domain-containing protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - iutA / iroD / iroC / iroB / rmpA2 / iucA / iucB / iucC / iucD / iutA / iroD / iroC 1..228102 228102
- flank IS/Tn - - 194600..195580 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(6-129)

Antitoxin

(7-47)


Sequences


Toxin        


Download         Length: 148 a.a.        Molecular weight: 16006.74 Da        Isoelectric Point: 9.3642

>T241997 WP_004213072.1 NZ_CP095429:193292-193735 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Download         Length: 444 bp

>T241997 NZ_OU943336:3342256-3342474 [Salmonella enterica subsp. enterica serovar Typhi]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8677.73 Da        Isoelectric Point: 4.4563

>AT241997 WP_004213073.1 NZ_CP095429:193065-193295 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT241997 NZ_OU943336:3341854-3342228 [Salmonella enterica subsp. enterica serovar Typhi]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB Q6U619


Antitoxin

Source ID Structure
AlphaFold DB Q6U620

References