Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 16673..17583 Replicon plasmid pIM007_vir
Accession NZ_CP095429
Organism Klebsiella pneumoniae strain IM007

Toxin (Protein)


Gene name - Uniprot ID A0A663AYG0
Locus tag MWR71_RS27780 Protein ID WP_004026354.1
Coordinates 17113..17583 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6P1V3Q9
Locus tag MWR71_RS27775 Protein ID WP_004026357.1
Coordinates 16673..17116 (+) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MWR71_RS27745 (MWR71_27705) 11691..12212 + 522 WP_004213635.1 ferric citrate uptake sigma factor FecI -
MWR71_RS27750 (MWR71_27710) 12209..13162 + 954 Protein_12 fec operon regulator FecR -
MWR71_RS27755 (MWR71_27715) 13249..15375 + 2127 WP_004213640.1 TonB-dependent Fe(3+) dicitrate receptor FecA -
MWR71_RS27760 (MWR71_27720) 15450..15575 + 126 Protein_14 Fe3+-citrate ABC transporter substrate-binding protein -
MWR71_RS27765 (MWR71_27725) 15750..15971 + 222 WP_004213643.1 DUF2188 domain-containing protein -
MWR71_RS27770 (MWR71_27730) 16069..16572 + 504 Protein_16 DUF4113 domain-containing protein -
MWR71_RS27775 (MWR71_27735) 16673..17116 + 444 WP_004026357.1 DUF2384 domain-containing protein Antitoxin
MWR71_RS27780 (MWR71_27740) 17113..17583 + 471 WP_004026354.1 RES family NAD+ phosphorylase Toxin
MWR71_RS27785 (MWR71_27745) 17732..18429 + 698 WP_223175001.1 IS1-like element IS1A family transposase -
MWR71_RS27790 (MWR71_27750) 19815..20465 - 651 WP_068893702.1 DUF1173 family protein -
MWR71_RS27795 (MWR71_27755) 20498..20764 - 267 WP_223175074.1 DUF1173 family protein -
MWR71_RS27800 (MWR71_27760) 20942..21436 + 495 WP_004212794.1 thermonuclease family protein -
MWR71_RS27805 (MWR71_27765) 21773..22171 + 399 WP_004212796.1 H-NS family nucleoid-associated regulatory protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - iutA / iroD / iroC / iroB / rmpA2 / iucA / iucB / iucC / iucD / iutA / iroD / iroC 1..228102 228102


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17515.79 Da        Isoelectric Point: 4.6155

>T241995 WP_004026354.1 NZ_CP095429:17113-17583 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T241995 NZ_OU943336:c975894-975367 [Salmonella enterica subsp. enterica serovar Typhi]
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGGAAAACCCACAATCGAATGAATTTTGATTGTGGGGATGCCGATCT
GAACCAATTCCTGCAACGCCATGCACGACAAAATCATGAGAAAGGGACAACAAAAACCTACGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCACGGCTTTTACTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATCAGCAAA
GGATTAGGGAGATATGATGTGCCAGTGTTTCGTCTGGGTCGTTTAGCCGTAGACAAATCTATGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAGGCGGCTTTGCAGGTCGGTGGCGTAGCCCTGCTTATTGATG
CCAAAAATAAACAGGTCTGCGACTGGTACAAAGGATTTGGCGCAGTACCATTAAACGATCAACCCCTTTCCTTGTTGCTG
TCGTTTAAAACGCTTTATGCTGCTTTATCTGCATCTGGTAGGTTATGA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16480.80 Da        Isoelectric Point: 10.2498

>AT241995 WP_004026357.1 NZ_CP095429:16673-17116 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT241995 NZ_OU943336:c976181-975891 [Salmonella enterica subsp. enterica serovar Typhi]
ATGAAAACCATGCCTCAGATAGCTATAGAATCTAACGAAAGATTGTCGTTACGTGTATCGACGGATGCCAAAAAGTTGAT
AGTCCGCGCTGCGGCAATCCAGCAAACCAACCTGACGGATTTTGTGGTCAGTAACGTACTACCTGTCGCACAGAAGATTG
TTGATGCCGCCGAACGGGTATATCTGACGGAGCGTGATACTCAAATGATCATGGAAATTTTAGATAACCCGCCAGCGCCG
AATGAAAAGCTTCTTGCCGCTGCTTTCGCCCTGCCGGACATGAAGAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AYG0

Antitoxin

Source ID Structure
AlphaFold DB A0A6P1V3Q9

References