Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 3182543..3183353 Replicon chromosome
Accession NZ_CP095428
Organism Klebsiella pneumoniae strain IM007

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag MWR71_RS15755 Protein ID WP_002887280.1
Coordinates 3182543..3183076 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag MWR71_RS15760 Protein ID WP_002887278.1
Coordinates 3183087..3183353 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MWR71_RS15750 (MWR71_15725) 3181374..3182495 + 1122 WP_002887282.1 cupin domain-containing protein -
MWR71_RS15755 (MWR71_15730) 3182543..3183076 - 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
MWR71_RS15760 (MWR71_15735) 3183087..3183353 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
MWR71_RS15765 (MWR71_15740) 3183456..3184889 - 1434 WP_002887275.1 Cu(+)/Ag(+) sensor histidine kinase -
MWR71_RS15770 (MWR71_15745) 3184879..3185562 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
MWR71_RS15775 (MWR71_15750) 3185734..3187119 + 1386 WP_002887267.1 efflux transporter outer membrane subunit -
MWR71_RS15780 (MWR71_15755) 3187137..3187481 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T241987 WP_002887280.1 NZ_CP095428:c3183076-3182543 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T241987 NZ_OU912926:2050085-2050579 [Candidatus Nitrotoga arctica]
ATGAGTTTGCAACTCAACGCACCTCAGCCACTCGCCACCACCCACATTTTGGATCGATTTGAGTGCGGCGAGGGCGTTCT
CGACGAGTGGCTCAAGCGCCGCGCAATGGTCAACCAAATGAGCGGAGCTAGCCGCACGTTTGTAGTGGCGGATCAGGATA
GTCATGTTTACGGCTACTATGCCATGGCTGCGGGCGCTGTCTCGCATCAGATGGCAACCAGTTCTGTGCGTCGAAACATG
CCCGATCCCGTTCCCGTAATGGTTCTGGCTCGGCTCGCGGTTGACCACCATGCCCAAGGCATTAAGCTTGGCGCATCCCT
ACTGCAAGACGCCGTCAACCGGGCTGTGGTGGTTTCTCAGAACGCCGGTGTCCGAGCTCTGCTGGTGCATGCACTTCACG
ACCGCGCCAAGGAGTTCTACGAGCACTACGGTTTTCAGTCGTCGCCGTTGCATCCGATGACGCTGATGCTGAGATTGAAT
ATCGGCAAGGCATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT241987 WP_002887278.1 NZ_CP095428:c3183353-3183087 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT241987 NZ_OU912926:2049816-2050088 [Candidatus Nitrotoga arctica]
ATGCGCGACGCCGCCATCAACCTACGGGCTTTGCCCGAGCAACGAGATTTAATTGATCACGCTGCAACTCTGCTAGGCAA
GAACCGTTCGGATTTCATGCTCGAGGCTGCCTGCTCAGCCGCGCAGGCGGTAATACTTGACCAGGTTTTTTTCAGCCTGA
ATACCGACAAGTTCCAGCAGTTCACGGCAATGCTCGACGCCCCGCCCAGCCCAAATCCAGGGCTTGAGCGCCTCATGGCC
GTGACAGCTCCTTGGGCCACCGTCAAAGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References