Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2568574..2569193 Replicon chromosome
Accession NZ_CP095428
Organism Klebsiella pneumoniae strain IM007

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag MWR71_RS12805 Protein ID WP_002892050.1
Coordinates 2568975..2569193 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag MWR71_RS12800 Protein ID WP_002892066.1
Coordinates 2568574..2568948 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MWR71_RS12790 (MWR71_12765) 2563726..2564919 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
MWR71_RS12795 (MWR71_12770) 2564942..2568088 + 3147 WP_020326861.1 multidrug efflux RND transporter permease subunit AcrB -
MWR71_RS12800 (MWR71_12775) 2568574..2568948 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
MWR71_RS12805 (MWR71_12780) 2568975..2569193 + 219 WP_002892050.1 HHA domain-containing protein Toxin
MWR71_RS12810 (MWR71_12785) 2569352..2569918 + 567 WP_002892030.1 maltose O-acetyltransferase -
MWR71_RS12815 (MWR71_12790) 2569890..2570030 - 141 WP_004147370.1 hypothetical protein -
MWR71_RS12820 (MWR71_12795) 2570051..2570521 + 471 WP_002892026.1 YlaC family protein -
MWR71_RS12825 (MWR71_12800) 2570496..2571947 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
MWR71_RS12830 (MWR71_12805) 2572048..2572746 + 699 WP_002892021.1 GNAT family protein -
MWR71_RS12835 (MWR71_12810) 2572743..2572883 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
MWR71_RS12840 (MWR71_12815) 2572883..2573146 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T241986 WP_002892050.1 NZ_CP095428:2568975-2569193 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T241986 NZ_OU912926:1816247-1816669 [Candidatus Nitrotoga arctica]
ATGAGCTGGCTGCTGGATACCTGCGCGCTTTCCGAATACGCCAAAAAGGCCCCCACACCGAAAGTTATCGCCTGGCTGGA
TGAACAAGACGAAACCAGCCTGTTCATCAGCGTCATCACGCTGGGTGAAATCGAAAAAGGCATCCTCAAACTACGCGCAA
ATGACCCGCACCGCAGCCAGAAACTCACTGCTTGGCTGGGTAAGGTGGAGCAGCGCTTTGCTGGGCGCATCCTGCCGCTC
AATGCCGCTGCCCTTCACGTATGGGCGCAAATCACCGCACACTCCGAACTGACTGGACAGCCCATGCCGGTGATGGACGG
CCTGCTCATGGCCACTGCCCAATGCCACGGCTTCACCGTGGTCACCCGTTACGTGCAGGATTTCGCGATGTACCCGCAAG
TGTTTAATCCTTGGGCGTTGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT241986 WP_002892066.1 NZ_CP095428:2568574-2568948 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT241986 NZ_OU912926:1815999-1816250 [Candidatus Nitrotoga arctica]
ATGCATAGGGAATGGCAACTGCAAAAAGCGAAAGGCAACTTCAGTCAGCTTATCAAGGAGGCGGTGAGTGGCGATGCCCA
AGTAGTTACGGTGCACGGAAAACCCACGGCGGTAATTGTTTCGGCTGAAGAATACGCTCGACTCACCCGCCACCGAACCA
AGCTCTCCAGCGCACTGCTGCGTCCCGATCTCGCAGCCGAAGATCTGGATATTTCCCGCAGCCGCGACGCCGGGCGCGAC
ATCGATCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References