Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | vagCD/VapC-VagC |
| Location | 56553..57196 | Replicon | plasmid pIM029_KPC |
| Accession | NZ_CP095427 | ||
| Organism | Klebsiella pneumoniae strain IM029 | ||
Toxin (Protein)
| Gene name | vagD | Uniprot ID | D8L2J9 |
| Locus tag | MWR69_RS28270 | Protein ID | WP_001044770.1 |
| Coordinates | 56553..56969 (-) | Length | 139 a.a. |
Antitoxin (Protein)
| Gene name | vagC | Uniprot ID | D5KTK7 |
| Locus tag | MWR69_RS28275 | Protein ID | WP_001261282.1 |
| Coordinates | 56966..57196 (-) | Length | 77 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| MWR69_RS28250 (51796) | 51796..52800 | + | 1005 | WP_000427619.1 | IS110-like element IS5075 family transposase | - |
| MWR69_RS28260 (53910) | 53910..54932 | - | 1023 | WP_000361404.1 | helicase UvrD | - |
| MWR69_RS28265 (54917) | 54917..56479 | - | 1563 | WP_004206609.1 | AAA family ATPase | - |
| MWR69_RS28270 (56553) | 56553..56969 | - | 417 | WP_001044770.1 | type II toxin-antitoxin system VapC family toxin | Toxin |
| MWR69_RS28275 (56966) | 56966..57196 | - | 231 | WP_001261282.1 | type II toxin-antitoxin system VapB family antitoxin | Antitoxin |
| MWR69_RS28280 (57153) | 57153..57614 | + | 462 | WP_014343465.1 | hypothetical protein | - |
| MWR69_RS28285 (57775) | 57775..58719 | + | 945 | WP_011977810.1 | hypothetical protein | - |
| MWR69_RS28290 (58756) | 58756..59148 | + | 393 | WP_011977811.1 | hypothetical protein | - |
| MWR69_RS28295 (59206) | 59206..59727 | + | 522 | WP_013214008.1 | hypothetical protein | - |
| MWR69_RS28300 (59773) | 59773..59976 | + | 204 | WP_011977813.1 | hypothetical protein | - |
| MWR69_RS28305 (60006) | 60006..61010 | + | 1005 | WP_011977814.1 | hypothetical protein | - |
| MWR69_RS28310 (61194) | 61194..61973 | + | 780 | WP_013214009.1 | site-specific integrase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Mobilizable plasmid | rmtB / fosA3 / blaKPC-2 / blaSHV-12 | - | 1..120647 | 120647 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 139 a.a. Molecular weight: 15106.52 Da Isoelectric Point: 7.1084
>T241976 WP_001044770.1 NZ_CP095427:c56969-56553 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR
Download Length: 417 bp
>T241976 NZ_OU907312:c566119-565613 [unidentified bacterial endosymbiont]
TTGATTATTTCATCCCCTGTATTGCTAACAAGTCAGCATCGAACTGAATTATTTTTCTCTGGTGTAGAGAGCTTGGATCT
CTGGCTCAAAAAGCGGGCCGTAAAAAATCAAAACACAGGGGCTTCACGGACTTTTGTGGCTTGCGACGGAGAAAGCGTAC
TGGCCTATTATGCACTGGCTTCTAGCGCCATTGCTGTTTCTGAAACATTTGGACGTTTTGGCCGCAACATGCCAGATCCC
ATTCCTGTCGTTATGCTTGGGAGACTAGCAGTAGATAAAAGTTTGCATGGAAACGGCATAGGAAGAGCACTGGTACGAGA
TGCTTGTCACCGAGTTATACAAGCTGCTGATTCTATAGGGGTCCGTGGAGTTATCGTACAAGCATTATCCGTTGAAGCAA
AAAAATTTTACAATCGGGTGGGCTTTGAAGCTTCTCCACTTAATCCCATGATACTCATGGTCACACTCACTGATTTAAAA
GCAAGTATTCAAAAAACAATCAATTAA
TTGATTATTTCATCCCCTGTATTGCTAACAAGTCAGCATCGAACTGAATTATTTTTCTCTGGTGTAGAGAGCTTGGATCT
CTGGCTCAAAAAGCGGGCCGTAAAAAATCAAAACACAGGGGCTTCACGGACTTTTGTGGCTTGCGACGGAGAAAGCGTAC
TGGCCTATTATGCACTGGCTTCTAGCGCCATTGCTGTTTCTGAAACATTTGGACGTTTTGGCCGCAACATGCCAGATCCC
ATTCCTGTCGTTATGCTTGGGAGACTAGCAGTAGATAAAAGTTTGCATGGAAACGGCATAGGAAGAGCACTGGTACGAGA
TGCTTGTCACCGAGTTATACAAGCTGCTGATTCTATAGGGGTCCGTGGAGTTATCGTACAAGCATTATCCGTTGAAGCAA
AAAAATTTTACAATCGGGTGGGCTTTGAAGCTTCTCCACTTAATCCCATGATACTCATGGTCACACTCACTGATTTAAAA
GCAAGTATTCAAAAAACAATCAATTAA
Antitoxin
Download Length: 77 a.a. Molecular weight: 8756.83 Da Isoelectric Point: 4.4692
>AT241976 WP_001261282.1 NZ_CP095427:c57196-56966 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL
Download Length: 231 bp
>AT241976 NZ_OU907312:c566406-566116 [unidentified bacterial endosymbiont]
ATGTCTATTTTAAGAGAATTACCAAATAAACGTGAGACCTTGAATCTGCGTATTAAACCAGATGAGCGTAATTTGATTGA
TTACGCTGCTAAAATTCAGGGAAAAAATCGTACAGATTTTATTCTAGAATCGGCACGGCTAGCCGCTGAGGAAACCTTGA
TCAACCAAGTTATCATGACAGCCACTCCAAAAGCGTATGGTGATTTTTTTAAACGGTTAGAAATGCCTCCTCTTCCTAAC
GATCGCTTGTGCACTATGATGCAAACACCAGCTCCCTGGGATAAAATTTGA
ATGTCTATTTTAAGAGAATTACCAAATAAACGTGAGACCTTGAATCTGCGTATTAAACCAGATGAGCGTAATTTGATTGA
TTACGCTGCTAAAATTCAGGGAAAAAATCGTACAGATTTTATTCTAGAATCGGCACGGCTAGCCGCTGAGGAAACCTTGA
TCAACCAAGTTATCATGACAGCCACTCCAAAAGCGTATGGTGATTTTTTTAAACGGTTAGAAATGCCTCCTCTTCCTAAC
GATCGCTTGTGCACTATGATGCAAACACCAGCTCCCTGGGATAAAATTTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0H3GYM2 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0H3GZG3 |