Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5548320..5548945 Replicon chromosome
Accession NZ_CP095426
Organism Klebsiella pneumoniae strain IM029

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag MWR69_RS27510 Protein ID WP_002882817.1
Coordinates 5548320..5548703 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag MWR69_RS27515 Protein ID WP_004150355.1
Coordinates 5548703..5548945 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MWR69_RS27495 (MWR69_27465) 5545686..5546588 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
MWR69_RS27500 (MWR69_27470) 5546585..5547220 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
MWR69_RS27505 (MWR69_27475) 5547217..5548146 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
MWR69_RS27510 (MWR69_27480) 5548320..5548703 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
MWR69_RS27515 (MWR69_27485) 5548703..5548945 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
MWR69_RS27520 (MWR69_27490) 5549150..5550067 + 918 WP_002882812.1 alpha/beta hydrolase -
MWR69_RS27525 (MWR69_27495) 5550081..5551022 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
MWR69_RS27530 (MWR69_27500) 5551067..5551504 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
MWR69_RS27535 (MWR69_27505) 5551501..5552361 - 861 WP_002882807.1 virulence factor BrkB family protein -
MWR69_RS27540 (MWR69_27510) 5552355..5552954 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T241968 WP_002882817.1 NZ_CP095426:c5548703-5548320 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T241968 NZ_OU701462:3754-4458 [Campylobacter upsaliensis]
ATGTGGGATACCCAAGATACATATACGGATATTGATACATTTTTACTCTCCACAAATAAGGTTAATGCAACTTCATTAAG
TGATTTAGAACACAAAGAAAGAATACTCGCTCAACTAGCAATGCAAGAACTCAGCAAAAATGAAATAAAAGGTAATTTTA
ACTACGAACACCTAAAAGCAATTCATCAATTTATTTTTAAAGATATTTACACTTGGGCTGGAAAAGACAGATATGAAGAA
AAATTTTTTAAGGCTTTACATAAAGGCGATTCTCAATTTTGTGTTGGCGTTTTAATCCCACAAGAATCAAAAAGAATATT
TGATGGTTTAATGGAAAAAGAATTGCTAAAAACTTGCAAAAATGATGAACAATTTATCAAAGAATTGGCAAATTTTATGG
CTGATTTAAACGCTATGCATCCATTTTGCGAGGGTAATGGACGCACTCAAAGAATTTTTATTAATCAACTTGCAAAAAAT
GCTGGATATGAACTTGATTTGAACTTAACTCCAAAAGAAATTATGCTTCGAGCTTCTATTGAAGCAATGAATCATCAAAA
TAGCTCTTTAGAAAAAATAATCAAAAACAATTTAAAGAAAATTGATATGTCTAAACAGAGGGAAATCGAAGCGATGCAAG
AGGCGGTTAAAAAAGCTTCGCAAAATCTTAAATCTAAAGATAAGAGCAAAGATTTAGATAGATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT241968 WP_004150355.1 NZ_CP095426:c5548945-5548703 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT241968 NZ_OU701462:3515-3754 [Campylobacter upsaliensis]
ATGTTGCAAAATTGTAAAGAAGTTTATAGCTTAGAAGAAAGCCTAAATATTTTAAATAAATATGCAAATAATCTCACACC
AAGACAATACGATAATATTAAGTCTATTATATGTAGCTTTGCCATAGAAAATATGCATCTCAATGAAGAAGATATTTTAA
ACGGCATAGCCATTCAAAAAGGCGATAAGAGTGTTTTAGAAATTACTCAAAATTATAAAGCTCAGTGGGGTTGCCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References